Aberrant P-cadherin expression is associated to aggressive feline mammary carcinomasReportar como inadecuado

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BMC Veterinary Research

, 10:270



BackgroundCadherins are calcium-dependent cell-to-cell adhesion glycoproteins playing a critical role in the formation and maintenance of normal tissue architecture. In normal mammary gland, E-cadherin is expressed by luminal epithelial cells, while P-cadherin is restricted to myoepithelial cells. Changes in the expression of classical E- and P-cadherins have been observed in mammary lesions and related to mammary carcinogenesis. P-cadherin and E-cadherin expressions were studied in a series of feline normal mammary glands, hyperplastic-dysplastic lesions, benign and malignant tumours by immunohistochemistry and double-label immunofluorescence.

ResultsIn normal tissue and in the majority of hyperplastic-dysplastic lesions and benign tumours, P-cadherin was restricted to myoepithelial cells, while 80% of the malignant tumours expressed P-cadherin in luminal epithelial cells. P-cadherin expression was significantly related to high histological grade of carcinomas p <0.0001, tumour necrosis p = 0.001, infiltrative growth p = 0.0051, and presence of neoplastic emboli p = 0.0401. Moreover, P-cadherin positive carcinomas had an eightfold likelihood of developing neoplastic emboli than negative tumours. Cadherins expression profile in high grade and in infiltrative tumours was similar, the majority expressing P-cadherin, regardless of E-cadherin expression status. The two cadherins were found to be co-expressed in carcinomas with aberrant P-cadherin expression and preserved E-cadherin.

ConclusionsThe results demonstrate a relationship between P-cadherin expression and aggressive biological behaviour of feline mammary carcinomas, suggesting that P-cadherin may be considered an indicator of poor prognosis in this animal species. Moreover, it indicates that, in queens, the aberrant expression of P-cadherin is a better marker of mammary carcinomas aggressive behaviour than the reduction of E-cadherin expression. Further investigation with follow-up studies in feline species should be conducted in order to evaluate the prognostic value of P-cadherin expression in E-cadherin positive carcinomas.

KeywordsFeline mammary tumours Invasion P-cadherin E-cadherin AbbreviationsHandEHaematoxylin and eosin

WHOWorld health organization


TBSTris-buffered saline


BSABovine serum albumin

PP-cadherin positive

PP-cadherin negative

EPreserved E-cadherin

EReduced E-cadherin

PBSPhosphate buffered saline

DIFDouble-label immunofluorescence

Electronic supplementary materialThe online version of this article doi:10.1186-s12917-014-0270-z contains supplementary material, which is available to authorized users.

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Autor: Ana Catarina Figueira - Catarina Gomes - Joana Tavares de Oliveira - Hugo Vilhena - Júlio Carvalheira - Augusto JF de Ma

Fuente: https://link.springer.com/

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