Leading-process actomyosin coordinates organelle positioning and adhesion receptor dynamics in radially migrating cerebellar granule neuronsReportar como inadecuado




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Neural Development

, 9:26

First Online: 02 December 2014Received: 12 June 2014Accepted: 26 September 2014

Abstract

BackgroundDuring brain development, neurons migrate from germinal zones to their final positions to assemble neural circuits. A unique saltatory cadence involving cyclical organelle movement e.g., centrosome motility and leading-process actomyosin enrichment prior to nucleokinesis organizes neuronal migration. While functional evidence suggests that leading-process actomyosin is essential for centrosome motility, the role of the actin-enriched leading process in globally organizing organelle transport or traction forces remains unexplored.

ResultsWe show that myosin ii motors and F-actin dynamics are required for Golgi apparatus positioning before nucleokinesis in cerebellar granule neurons CGNs migrating along glial fibers. Moreover, we show that primary cilia are motile organelles, localized to the leading-process F-actin-rich domain and immobilized by pharmacological inhibition of myosin ii and F-actin dynamics. Finally, leading process adhesion dynamics are dependent on myosin ii and F-actin.

ConclusionsWe propose that actomyosin coordinates the overall polarity of migrating CGNs by controlling asymmetric organelle positioning and cell-cell contacts as these cells move along their glial guides.

AbbreviationsCGNCerebellar granule neuron

FRAPFluorescence recovery after photobleaching

KO2Kusibira orange

MGEMedial ganglionic eminence

RFPRed fluorescent protein

TFPTeal fluorescent protein

UTRCH-ABDUtrophin actin-binding domain

YFPYellow fluorescent protein.

Electronic supplementary materialThe online version of this article doi:10.1186-1749-8104-9-26 contains supplementary material, which is available to authorized users.

Joseph S Ramahi, Mahmut Karakaya contributed equally to this work.

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Autor: Niraj Trivedi - Joseph S Ramahi - Mahmut Karakaya - Danielle Howell - Ryan A Kerekes - David J Solecki

Fuente: https://link.springer.com/



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