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BMC Infectious Diseases

, 14:677

Viral diseases


BackgroundThe genital skin of males hosts a diversity of HPV genotypes and uncharacterized HPV genotypes. Previously we demonstrated that a specific viral genotype was not identified in 14% of all genital specimens i.e., HPV unclassified specimens using the Roche Linear Array method. Our goal was to identify and assess the prevalence of individual HPV types among genital HPV unclassified specimens collected in the HIM Study population, at enrollment, and examine associations with socio-demographic and behavioral characteristics.

MethodsGenital skin specimens of men that were considered unclassified HPV PCR positive, no genotype specified at enrollment were typed by sequencing amplified PGMY09-11 products or cloning of PGMY-GP+ nested amplicons followed by sequencing. PGMY-GP+ negative specimens were further analyzed using FAP primers. HPV type classification was conducted through comparisons with sequences in the GenBank database.

ResultsReadable nucleotide sequences were generated for the majority of previously unclassified specimens 66%, including both characterized 77% and yet uncharacterized 23% HPV types. Of the characterized HPV types, most 73% were Beta β-HPVs, primarily from β-1 and β-2 species, followed by Alpha α-HPVs 20%. Smokers current and former were significantly more likely to have an α-HPV infection, compared with any other genus; no other factors were associated with specific HPV genera or specific β-HPV species.

ConclusionsMale genital skin harbor a large number of β-HPV types. Knowledge concerning the prevalence of the diverse HPV types in the men genital is important to better understand the transmission of these viruses.

KeywordsHuman papillomavirus Cutaneous HPV Males HIM Study Prevalence Prospective study AbbreviationsHPVHuman papillomavirus

HIM StudyHPV Infection in Men Study




Electronic supplementary materialThe online version of this article doi:10.1186-s12879-014-0677-y contains supplementary material, which is available to authorized users.

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Autor: Laura Sichero - Christine M Pierce Campbell - William Fulp - Silvaneide Ferreira - João S Sobrinho - Maria Luiza Baggio

Fuente: https://link.springer.com/

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