Babesia behnkei sp. nov., a novel Babesia species infecting isolated populations of Wagner’s gerbil, Dipodillus dasyurus, from the Sinai Mountains, EgyptReportar como inadecuado




Babesia behnkei sp. nov., a novel Babesia species infecting isolated populations of Wagner’s gerbil, Dipodillus dasyurus, from the Sinai Mountains, Egypt - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Parasites and Vectors

, 7:572

First Online: 09 December 2014Received: 31 July 2014Accepted: 25 November 2014

Abstract

BackgroundAlthough a number of new species of Babesia-Theileria have been described recently, there are still relatively few reports of species from Africa. In this study based on the evaluation of morphology and phylogenetic relationships, we describe a novel species from Wagner’s gerbil, Babesia behnkei n. sp.

MethodsRodents n = 1021 were sampled in four montane valleys wadies in 2000, 2004, 2008 and 2012 in the Sinai Mountains, Egypt. The overall prevalence of Babesia spp. was highest in the Wagner’s gerbil Dipodillus dasyurus; 38.7% in comparison to the prevalence in the spiny mice species, Acomys dimidiatus and A. russatus. Morphological investigations were conducted for the comparison of trophozoites of the novel species of Babesia with the B. microti King’s 67 reference strain. Thirty-two isolates derived from D. dasyurus over a 9 year period 2004-2012 from two wadies 29 isolates from Wadi Gebel and 3 from Wadi El-Arbaein were investigated by microscopic, molecular and phylogenetic analysis. A near-full-length sequence of the 18S rRNA gene and the second internal transcribed spacer ITS2 region were amplified, sequenced and used for the construction of phylogenetic trees.

ResultsA novel species of Babesia was identified in two isolated populations of D. dasyurus. Phylogenetic analysis of 18S rDNA and ITS2 sequences revealed that B. behnkei n. sp. is most closely related to B. lengau from cheetahs from South Africa and to Nearctic species found only in North America the pathogenic B. duncani and B. conradae and that it is more distant to the cosmopolitan rodent parasite B. microti. Trophozoites of B. behnkei were smaller and less polymorphic than trophozoites of B. microti.

ConclusionBabesia behnkei n. sp. is a novel species of the ‘Duncani group’ maintained in isolated populations of Dipodillus dasyurus occurring in the Sinai Mountains of Egypt.

KeywordsBabesia Dipodillus dasyurus 18S rDNA ITS2 Phylogenetic analysis Sinai Egypt Electronic supplementary materialThe online version of this article doi:10.1186-s13071-014-0572-9 contains supplementary material, which is available to authorized users.

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Autor: Anna Bajer - Mohammed Alsarraf - Małgorzata Bednarska - Eman ME Mohallal - Ewa J Mierzejewska - Jolanta Behnke-Borowczyk

Fuente: https://link.springer.com/







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