Biocidal Properties of a Glycosylated Surface: Sophorolipids on Au111Reportar como inadecuado

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1 LRS - Laboratoire de Réactivité de Surface 2 Ghent University 3 SMiLES - Spectroscopie, Modélisation, Interfaces pour L-Environnement et la Santé LCMCP - Laboratoire de Chimie de la Matière Condensée de Paris

Abstract : Classical antibacterial surfaces usually involve antiadhesive and-or biocidal strategies. Glycosylated surfaces are usually used to prevent biofilm formation via antiadhesive mechanisms. We report here the first example of a glycosylated surface with biocidal properties created by the covalent grafting of sophorolipids a sophorose unit linked by a glycosidic bond to an oleic acid through a self-assembled monolayer SAM of short aminothiols on gold 111 surfaces. The biocidal effect of such surfaces on Gram+ bacteria was assessed by a wide combination of techniques including microscopy observations, fluorescent staining, and bacterial growth tests. About 50% of the bacteria are killed via alteration of the cell envelope. In addition, the roles of the sophorose unit and aliphatic chain configuration are highlighted by the lack of activity of substrates modified, respectively, with sophorose-free oleic acid and sophorolipid-derivative having a saturated aliphatic chain. This system demonstrates thus the direct implication of a carbohydrate in the destabilization and disruption of the bacterial cell envelope.

Keywords : killing-by-contact biocidal mechanism sophorolipid glycolipid surface functionalization self-assembled monolayer antibacterial coating

Autor: Claire Valotteau - Christophe Calers - Sandra Casale - Jan Berton - Christian V. Stevens - Florence Babonneau - Claire-Marie Prad



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