Attenuation of inflammatory-mediated neurotoxicity by Saururus chinensis extract in LPS-induced BV-2 microglia cells via regulation of NF-κB signaling and anti-oxidant propertiesReportar como inadecuado




Attenuation of inflammatory-mediated neurotoxicity by Saururus chinensis extract in LPS-induced BV-2 microglia cells via regulation of NF-κB signaling and anti-oxidant properties - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Complementary and Alternative Medicine

, 14:502

Basic research

Abstract

BackgroundA Saururus chinensis Baill SC has been used by Native Americans, early colonists and practitioners of Korean traditional medicine for treating several diseases including cancer, rheumatoid arthritis and edema. The objective of this study was to evaluate the effects of SC extract in lipopolysaccharide LPS-stimulated neuroinflammatory responses in BV-2 microglial cells.

MethodsThe effects of SC on the LPS–induced neuroinflammatory responses in BV-2 microglial cells were assessed by Western blotting, RT-PCR and immunofluorescence labeling techniques. DPPH and alkyl radical scavenging assay was performed to evaluate the anti-oxidant effects. Comparisons between groups were analyzed using one-way analysis of variance followed by Dunnett’s multiple comparisons test using GraphPad Prism V5.01 software.

ResultsPre-treatment with SC extract 1, 5 and 10 μg-mL significantly p < 0.001 at 10 μg-mL and concentration dependently inhibited LPS-induced production of nitric oxide NO, inducible NO synthase iNOS, cyclooxygenase-2 COX-2 and suppressed the inflammatory cytokine levels such as tumor necrosis factor-alpha and interleukin IL-6 in BV-2 microglial cells p < 0.001 at 10 μg-mL. Further, SC suppressed the nuclear factor-kappa B NF-κB activation by blocking the degradation of IκB-α. SC also exhibited profound anti-oxidant effects by scavenging 1, 1-diphenyl-2-picrylhydrazyl DPPH IC50: 0.055 mg-mL and alkyl radicals IC50: 0.349 mg-mL. High performance liquid chromatography finger printing analysis of SC revealed quercetin QCT as one of the major constituents compared with reference standard. QCT also inhibited the excessive release of NO, and inhibited the increased expressional levels of IL-6, iNOS and COX-2 in LPS-stimulated BV-2 cells.

ConclusionsOur results indicated that SC inhibited the LPS-stimulated neuroinflammatory responses in BV-2 microglia via regulation of NF-κB signaling. The antioxidant active constituents of SC might be partly involved in delivering such effects. Based on the traditional claims and our present results SC can be potentially used in treating inflammatory-mediated neurodegenerative diseases.

KeywordsMicroglia Saururus chinensis Quercetin LPS NF-κB Neurodegenerative disease AbbreviationsBSABovine Serum Albumin

COX-2Cyclooxygenase-2

DMEMDimethyl Sulfoxide

FBSFetal Bovine Serum

GAPDHGlyceraldehydes-3-Phosphate Dehydrogenase

HPLCHigh-performance liquid chromatography

IĸB-αInhibitory protein kappa B alpha

IL-1βInterleukin-1beta

IL-6Interleukin-6

iNOSInducible Nitric Oxide Synthase

LPSLipopolysaccharide

MTTp-nitrophenyl phosphate, and 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide

NF-ĸBNuclear factor kappa B

NONitric Oxide

PBSPhosphate-buffered saline

PDParkinson’s Disease

RT-PCRReverse transcription polymerase chain reaction

SDSSodium Dodecyl Sulfate

TNF-αTumor necrosis factor-α.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6882-14-502 contains supplementary material, which is available to authorized users.

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Autor: Byung-Wook Kim - Sushruta Koppula - Shin-Young Park - Jin-Woo Hwang - Pyo-Jam Park - Ji-Hong Lim - Dong-Kug Choi

Fuente: https://link.springer.com/







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