First co-expression of a lipase and its specific foldase obtained by metagenomicsReportar como inadecuado

First co-expression of a lipase and its specific foldase obtained by metagenomics - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Microbial Cell Factories

, 13:171

First Online: 16 December 2014Received: 06 October 2014Accepted: 20 November 2014


BackgroundMetagenomics is a useful tool in the search for new lipases that might have characteristics that make them suitable for application in biocatalysis. This paper reports the cloning, co-expression, purification and characterization of a new lipase, denominated LipG9, and its specific foldase, LifG9, from a metagenomic library derived from a fat-contaminated soil.

ResultsWithin the metagenomic library, the gene lipg9 was cloned jointly with the gene of the foldase, lifg9. LipG9 and LifG9 have 96% and 84% identity, respectively, with the corresponding proteins of Aeromonas veronii B565. LipG9 and LifG9 were co-expressed, both in N-truncated form, in Escherichia coli BL21DE3, using the vectors pET28a+ and pT7-7, respectively, and then purified by affinity chromatography using a Ni column HiTrap Chelating HP. The purified enzyme eluted from the column complexed with its foldase. The molecular masses of the N-truncated proteins were 32 kDa for LipG9, including the N-terminal His-tag with 6 residues, and 23 kDa for LifG9, which did not have a His-tag. The biochemical and kinetic characteristics of the purified lipase-foldase preparation were investigated. This preparation was active and stable over a wide range of pH values 6.5-9.5 and temperatures 10-40°C, with the highest specific activity, of 1500 U mg, being obtained at pH 7.5 at 30°C. It also had high specific activities against tributyrin, tricaprylin and triolein, with values of 1852, 1566 and 817 U mg, respectively. A phylogenetic analysis placed LipG9 in the lipase subfamily I.1. A comparison of the sequence of LipG9 with those of other bacterial lipases in the Protein Data Bank showed that LipG9 contains not only the classic catalytic triad Ser, Asp, His, with the catalytic Ser occurring within a conserved pentapeptide, Gly-His-Ser-His-Gly, but also a conserved disulfide bridge and a conserved calcium binding site. The homology-modeled structure presents a canonical α-β hydrolase folding type I.

ConclusionsThis paper is the first to report the successful co-expression of a lipase and its associated foldase from a metagenomic library. The high activity and stability of Lip-LifG9 suggest that it has a good potential for use in biocatalysis.

KeywordsLipases Metagenomics Biocatalysis Lipase-foldase co-expression Electronic supplementary materialThe online version of this article doi:10.1186-s12934-014-0171-7 contains supplementary material, which is available to authorized users.

Download fulltext PDF

Autor: Viviane Paula Martini - Arnaldo Glogauer - Marcelo Müller-Santos - Jorge Iulek - Emanuel Maltempi de Souza - David Alexan


Documentos relacionados