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Clinical Proteomics

, 10:11

First Online: 06 September 2013Received: 30 April 2013Accepted: 11 August 2013

Abstract

BackgroundArthritis refers to inflammation of joints and includes common disorders such as rheumatoid arthritis RA and spondyloarthropathies SpAs. These diseases differ mainly in terms of their clinical manifestations and the underlying pathogenesis. Glycoproteins in synovial fluid might reflect the disease activity status in the joints affected by arthritis; yet they have not been systematically studied previously. Although markers have been described for assisting in the diagnosis of RA, there are currently no known biomarkers for SpA.

Materials and methodsWe sought to determine the relative abundance of glycoproteins in RA and SpA by lectin affinity chromatography coupled to iTRAQ labeling and LC-MS-MS analysis. We also used ELISA to validate the overexpression of VCAM-1, one of the candidate proteins identified in this study, in synovial fluid from RA patients.

Results and discussionWe identified proteins that were previously reported to be overexpressed in RA including metalloproteinase inhibitor 1 TIMP1, myeloperoxidase MPO and several S100 proteins. In addition, we discovered several novel candidates that were overexpressed in SpA including Apolipoproteins C-II and C-III and the SUN domain-containing protein 3 SUN3. Novel molecules found overexpressed in RA included extracellular matrix protein 1 ECM1 and lumican LUM. We validated one of the candidate biomarkers, vascular cell adhesion molecule 1 VCAM1, in 20 RA and SpA samples using ELISA and confirmed its overexpression in RA p-value <0.01. Our quantitative glycoproteomic approach to study arthritic disorders should open up new avenues for additional proteomics-based discovery studies in rheumatological disorders.

KeywordsPannus Prognostic marker Endothelial dysfunction Synovium Biomarkers Electronic supplementary materialThe online version of this article doi:10.1186-1559-0275-10-11 contains supplementary material, which is available to authorized users.

Rakesh Sharma, Renu Goel contributed equally to this work.

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Autor: Mitali Bhattacharjee - Rakesh Sharma - Renu Goel - Lavanya Balakrishnan - Santosh Renuse - Jayshree Advani - Shantal Tankal

Fuente: https://link.springer.com/



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