Molecular characterization of T cell receptor beta variable in the peripheral blood T cell repertoire in subjects with active tuberculosis or latent tuberculosis infectionReportar como inadecuado




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BMC Infectious Diseases

, 13:423

Tuberculosis and other mycobacterial diseases

Abstract

BackgroundT cells are closely linked to the clinical manifestations of subjects with Mycobacterium tuberculosis MTB infection. T cell receptor beta variable TCRBV is a signal and indicative molecule on the membrane of T lymphocytes, reflecting the composition and specificity of T cells. The molecular profiles of TCRBV in peripheral blood mononuclear cells PBMCs and their subpopulations CD4 and CD8 T cells from subjects with active tuberculosis TB or latent TB infection LTBI have not been well described.

MethodsIn 42 subjects with active TB or LTBI, PMBCs and their subsets were separated and sorted. The molecular profiles of the TCRBV complementarity determining region 3 CDR3 in the three cell populations were investigated using our recently developed gene melting spectral pattern GMSP assay. The TCRBV members were then cloned and sequenced when their GMSP image profiles showed a single-peak.

ResultsThe average number of skewed TCRBV molecules in the CD4 cell subset was significantly higher than that in PBMCs and CD8 T cells. TCRBV12, BV13.1, BV13.2, and BV24 were expressed more prevalently than other TCRBV gene families in the three cell populations. In addition, relatively conserved amino acid motifs were identified in TCRBV5.1 and BV20 CDR3 in PBMCs and its subsets. The monoclonal TCRBV14 and BV23 expressed were different between active TB and LTBI subjects.

ConclusionsThese results indicate that the T cell immune response is complex and multi-specific in active TB and LTBI subjects. Analysis of TCRBV expression in CD4 T cells suggest that it could be useful in assessing the composition and status of circulating T cells. Furthermore, the expression of TCRBV14, BV23 and the sequencing of CDR3 amino acid motifs of TCRBV5.1, BV20 could be used in the differential diagnosis and treatment of subjects with active TB or LTBI.

AbbreviationsCDR3Complementarity determining region 3

GMSPGene melting spectral pattern

IGRAIFN-gamma release assay

LTBILatent TB infection

MTBMycobacterium tuberculosis

TBTuberculosis

TCRBVT cell receptor beta variable.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2334-13-423 contains supplementary material, which is available to authorized users.

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Autor: Jiezuan Yang - Jianqin He - Haijun Huang - Zhongkang Ji - Li Wei - Ping Ye - Kaijin Xu - Lanjuan Li

Fuente: https://link.springer.com/







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