Phenyl aldehyde and propanoids exert multiple sites of action towards cell membrane and cell wall targeting ergosterol in Candida albicansReportar como inadecuado




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AMB Express

, 3:54

First Online: 08 September 2013Received: 03 September 2013Accepted: 05 September 2013

Abstract

In the present study, two phyto-compounds phenyl aldehyde cinnamaldehyde and propanoid eugenol were selected to explore their modes of action against Candida albicans. Electron microscopy, flow cytometry and spectroscopic assays were employed to determine the targets of these compounds. Treatment of C. albicans CA04 with sub-MICs of cinnamaldehyde 50 μg-mL and eugenol 200 μg-mL indicated multiple sites of action including damages to cell walls, cell membranes, cytoplasmic contents and other membranous structures as observed under electron microscopy. Concentration and time dependent increase in the release of cytoplasmic contents accompanied with change in extracellular K concentration was recorded. Exposure of Candida cells at 4 × MIC of cinnaamldehyde and eugenol resulted in 40.21% and 50.90% dead cells, respectively as revealed by flow cytometry analysis. Treatment of Candida cells by cinnamaldehyde and eugenol at 0.5 × MIC showed 67.41% and 76.23% reduction in ergosterol biosynthesis, respectively. The binding assays reflected the ability of compounds to bind with the ergosterol. Our findings have suggested that the membrane damaging effects of phenyl aldehyde and propanoids class of compounds is attributed to their ability to inhibit ergosterol biosynthesis and simultaneously binding with ergosterol. Indirect or secondary action of these compounds on cell wall is also expected as revealed by electron microscopic studies.

KeywordsCandida albicans Electron microscopy Ergosterol Flow cytometry Electronic supplementary materialThe online version of this article doi:10.1186-2191-0855-3-54 contains supplementary material, which is available to authorized users.

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Autor: Mohd Sajjad Ahmad Khan - Iqbal Ahmad - Swaranjit Singh Cameotra

Fuente: https://link.springer.com/







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