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Chemistry Central Journal

, 7:152

Analytical Chemistry

Abstract

BackgroundA comparative study of the use of mean centering of ratio spectra and inverse least squares for the resolution of paracetamol, methylparaben, propylparaben, chlorpheniramine maleate and pseudoephedrine hydrochloride has been achieved showing that the two chemometric methods provide a good example of the high resolving power of these techniques. Method I is the mean centering of ratio spectra which depends on using the mean centered ratio spectra in four successive steps that eliminates the derivative steps and therefore the signal to noise ratio is improved. The absorption spectra of prepared solutions were measured in the range of 220–280 nm. Method II is based on the inverse least squares that depend on updating developed multivariate calibration model. The absorption spectra of the prepared mixtures in the range 230–270 nm were recorded.

ResultsThe linear concentration ranges were 0–25.6, 0–15.0, 0–15.0, 0–45.0 and 0–100.0 μg mL for paracetamol, methylparaben, propylparaben, chlorpheniramine maleate and pseudoephedrine hydrochloride, respectively. The mean recoveries for simultaneous determination were between 99.9-101.3% for the two methods. The two developed methods have been successfully used for prediction of five-component mixture in Decamol Flu syrup with good selectivity, high sensitivity and extremely low detection limit.

ConclusionNo published method has been reported for simultaneous determination of the five components of this mixture so that the results of the mean centering of ratio spectra method were compared with those of the proposed inverse least squares method. Statistical comparison was performed using t-test and F-ratio at P = 0.05. There was no significant difference between the results.

Open image in new windowKeywordsMethylparaben Propylparaben Chlorpheniramine Pseudoephedrine Mean centering ratio Inverse least square Spectroscopy Electronic supplementary materialThe online version of this article doi:10.1186-1752-153X-7-152 contains supplementary material, which is available to authorized users.

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Autor: Mahmoud Mohamed Issa - R’afat Mahmoud Nejem - Alaa Mohamed Abu Shanab - Nahed Talab Shaat

Fuente: https://link.springer.com/



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