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BMC Plant Biology

, 13:132

Biochemistry and physiology

Abstract

BackgroundAs the final stage of leaf development, leaf senescence may cause the decline of photosynthesis and gradual reduction of carbon assimilation, which makes it a possible limiting factor for crop yield. NACs are plant-specific transcription factors and some NACs have been confirmed to play important roles in regulating leaf senescence.

ResultsIn this study, we reported a member of the NAC transcription factor family named OsNAP whose expression is associated with leaf senescence, and investigated its preliminary function during the process of leaf senescence. The results of qRT-PCR showed that the OsNAP transcripts were accumulated gradually in response to leaf senescence and treatment with methyl jasmonic acid MeJA. A subcellular localization assay indicated that OsNAP is a nuclear-localized protein. Yeast one-hybrid experiments indicated that OsNAP can bind the NAC recognition site NACRS-like sequence. OsNAP-overexpressing transgenic plants displayed an accelerated leaf senescence phenotype at the grain-filling stage, which might be caused by the elevated JA levels and the increased expression of the JA biosynthesis-related genes LOX2 and AOC1, and showed enhanced tolerance ability to MeJA treatment at the seedling stage. Nevertheless, the leaf senescence process was delayed in OsNAP RNAi transgenic plants with a dramatic drop in JA levels and with decreased expression levels of the JA biosynthesis-related genes AOS2, AOC1 and OPR7.

ConclusionsThese results suggest that OsNAP acts as a positive regulator of leaf senescence and this regulation may occur via the JA pathway.

KeywordsChlorophyll Leaf senescence NAC JA Rice Oryza sativa L. AbbreviationsJAJasmonic acid

MeJAMethyl jasmonate acid

ORFOpen reading frame

WTWild-type

3-AT3-amino-1,2,4-triazole

TR domainTranscriptional regulatory TR domain

DAHDay after heading.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2229-13-132 contains supplementary material, which is available to authorized users.

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Autor: Yong Zhou - Weifeng Huang - Li Liu - Taiyu Chen - Fei Zhou - Yongjun Lin

Fuente: https://link.springer.com/



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