Involvement of ethylene biosynthesis and signalling in fruit set and early fruit development in zucchini squash Cucurbita pepoL.Reportar como inadecuado




Involvement of ethylene biosynthesis and signalling in fruit set and early fruit development in zucchini squash Cucurbita pepoL. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Plant Biology

, 13:139

Reproductive biology

Abstract

BackgroundWe have identified a kind of parthenocarpy in zucchini squash which is associated with an incomplete andromonoecy, i.e. a partial conversion of female into bisexual flowers. Given that andromonoecy in this and other cucurbit species is caused by a reduction of ethylene production in the female flower, the associated parthenocarpic development of the fruit suggested the involvement of ethylene in fruit set and early fruit development.

ResultsWe have compared the production of ethylene as well as the expression of 13 ethylene biosynthesis and signalling genes in pollinated and unpollinated ovaries-fruits of two cultivars, one of which is parthenocarpic Cavili, while the other is non-parthenocarpic Tosca. In the latter, unpollinated ovaries show an induction of ethylene biosynthesis and ethylene signal transduction pathway genes three days after anthesis, which is concomitant with the initiation of fruit abortion and senescence. Fruit set and early fruit development in pollinated flowers of both cultivars and unpollinated flowers of Cavili is coupled with low ethylene biosynthesis and signalling, which would also explain the partial andromonoecy in the parthenocarpic genotype. The reduction of ethylene production in the ovary cosegregates with parthenocarpy and partial andromonoecy in the selfing progeny of Cavili. Moreover, the induction of ethylene in anthesis by ethephon treatments reduced the percentage of bisexual parthenocarpic flowers in Cavili, while the inhibition of ethylene biosynthesis or response by AVG and STS treatments induces not only andromonoecy but also the parthenocarpic development of the fruit in both cultivars.

ConclusionsResults demonstrate that a reduction of ethylene production or signalling in the zucchini flower is able to induce fruit set and early fruit development, and therefore that ethylene is actively involved in fruit set and early fruit development. Auxin and TIBA treatments, inducing fruit set and early fruit development in this species, also inhibit ethylene production and the expression of ethylene biosynthesis and response genes. A model is presented that discusses the crosstalk between ethylene and auxin in the control of fruit set and early fruit development in zucchini squash.

KeywordsCucurbita pepo Fruit set Parthenocarpy Ethylene Gene expression Auxin AbbreviationsACC1-aminocyclopropane-1-carboxylic acid

ACOAcc oxidase

ACSAcc synthase

DPADays post anthesis

GAsGibberellins

1-MCP1- Methylcyclopropene

STSSilver thiosuphate

AVGAminoethoxyvinylglycine

NAA1-naphthalene acetic acid

TIBATriiodobenzoic acid

CTRConstitutive triple response

ERSEthylene response sensor

ETREthylene receptor

EIN3Ethylene insensitive3.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2229-13-139 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Cecilia Martínez - Susana Manzano - Zoraida Megías - Dolores Garrido - Belén Picó - Manuel Jamilena

Fuente: https://link.springer.com/



DESCARGAR PDF




Documentos relacionados