Ocular and neural distribution of feline herpesvirus-1 during active and latent experimental infection in catsReportar como inadecuado




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BMC Veterinary Research

, 9:185

Virology

Abstract

BackgroundHerpes simplex virus 1 HSV-1 and varicella zoster virus VZV cause extensive intra-ocular and neural infections in humans and are closely related to Felid herpes virus 1 FeHV-1. We report the extent of intra-ocular replication and the extent and morphological aspects of neural replication during the acute and latent phases of FeHV-1 infection. Juvenile, SPF cats were inoculated with FeHV-1. Additional cats were used as negative controls. Cats were euthanized on days 6, 10, and 30 post-inoculation.

ResultsFeHV-1 was isolated from the conjunctiva, cornea, uveal tract, retina, optic nerve, ciliary ganglion CG, pterygopalatine ganglion PTPG, trigeminal ganglion TG, brainstem, visual cortex, cerebellum, and olfactory bulb of infected cats during the acute phase, but not the cranial cervical ganglion CCG and optic chiasm. Viral DNA was detected in all tissues during acute infection by a real-time quantitative PCR assay. On day 30, viral DNA was detected in all TG, all CCG, and 2 PTPG. Histologically mild inflammation and ganglion cell loss were noted within the TG during acute, but not latent infection. Using linear regression, a strong correlation existed between clinical score and day 30 viral DNA copy number within the TG.

ConclusionsThe correlation between clinical score and day 30 viral DNA copy number suggests the severity of the acute clinical infection is related to the quantity of latent viral DNA. The histologic response was similar to that seen during HSV-1 or VZV infection. To the author’s knowledge this is the first report of FeHV-1 infection involving intraocular structures and autonomic ganglia.

KeywordsFeline Felid herpes virus1 FeHV-1 Latency Ganglia Eye AbbreviationsFeHV-1Felid herpesvirus 1

BHV-1Bovine herpesvirus 1

CCGCranial cervical ganglion

CGCiliary ganglion

HSV-1Herpes simplex virus 1

PIPost-inoculation

PTPGPterygopalatine ganglion

RT-PCRReal time polymerase chain reaction assay

TGTrigeminal ganglion

VIVirus isolation

VNVirus neutralizing

VZVVaricella zoster virus.

Electronic supplementary materialThe online version of this article doi:10.1186-1746-6148-9-185 contains supplementary material, which is available to authorized users.

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Autor: Wendy M Townsend - Susan Jacobi - Shih-Han Tai - Matti Kiupel - Annabel G Wise - Roger K Maes

Fuente: https://link.springer.com/







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