Histone deacetylase HDAC inhibitory and antiproliferative activities of phenolic-rich extracts derived from the rhizome of Hydnophytum formicarum Jack.: sinapinic acid acts as HDAC inhibitorReportar como inadecuado




Histone deacetylase HDAC inhibitory and antiproliferative activities of phenolic-rich extracts derived from the rhizome of Hydnophytum formicarum Jack.: sinapinic acid acts as HDAC inhibitor - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Complementary and Alternative Medicine

, 13:232

Basic research

Abstract

BackgroundThe rhizome of Hydnophytum formicarum Jack., a medicinal plant known in Thai as Hua-Roi-Roo, has been used in Thai traditional herbal medicine for treatment of cancer. We assessed the ability of its ethanolic and phenolic-rich extracts and its major phenolic compound, sinapinic acid, possessing histone deacetylase HDAC inhibitory activity to inhibit proliferation of 5 human cancer cell lines.

MethodsHeLa cells were used to study HDAC inhibitory activity of the extracts, sinapinic acid, and a well-known HDAC inhibitor sodium butyrate. Five human cancer cell lines and one non-cancer cell line were used to study antiproliferative activities of the plant extracts, sinapinic acid and sodium butyrate, comparatively.

ResultsResults indicated that ethanolic and phenolic-rich extracts of H. formicarum Jack. rhizome possessed both antiproliferative activity and HDAC inhibitory activity in HeLa cells. Sinapinic acid, despite its lower HDAC inhibitory activity than the well-known HDAC inhibitor sodium butyrate, inhibited the growth of HeLa and HT29 cells more effectively than sodium butyrate. However, sinapinic acid inhibited the growth of HCT116 and Jurkat cells less effectively than sodium butyrate. The non-cancer cell line Vero cells and breast cancer cell line MCF-7 cells appeared to be resistant to both sinapinic acid and sodium butyrate. The growth inhibitory effects of the ethanolic and phenolic-rich extracts and sinapinic acid in HeLa cells were mediated by induction of apoptosis.

ConclusionsThe results of this study support the efficacy of H. formicarum Jack. rhizome ethanolic and phenolic-rich extracts for the treatment of cervical cancer, colon cancer, and T- cell leukemia in an alternative medicine. Further studies of other active ingredients from this plant are needed.

KeywordsHydnophytum formicarum Jack HDAC inhibitors Anticancer activity Sinapinic acid AbbreviationsHDACHistone deacetylase

H. formicarum Jack.Hydnophytum formicarum Jack.

AUTAcid Urea-Triton-X-100

DMSODimethyl sulfoxide

NaBSodium butyrate

PIPropidium iodide.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6882-13-232 contains supplementary material, which is available to authorized users.

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Autor: Thanaset Senawong - Suwatchai Misuna - Somprasong Khaopha - Suporn Nuchadomrong - Prasan Sawatsitang - Chanokbhorn Phaosiri

Fuente: https://link.springer.com/







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