Cochaperonin CPN20 negatively regulates abscisic acid signaling in ArabidopsisReportar como inadecuado

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Plant Molecular Biology

, Volume 83, Issue 3, pp 205–218

First Online: 20 June 2013Received: 07 May 2013Accepted: 26 May 2013


Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit CHLH-ABAR positively regulates abscisic acid ABA signaling. Here, we investigated the functions of a CHLH-ABAR interaction protein, the chloroplast co-chaperonin 20 CPN20 in ABA signaling in Arabidopsis thaliana. We showed that down-expression of the CPN20 gene increases, but overexpression of the CPN20 gene reduces, ABA sensitivity in the major ABA responses including ABA-induced seed germination inhibition, postgermination growth arrest, promotion of stomatal closure and inhibition of stomatal opening. Genetic evidence supports that CPN20 functions downstream or at the same node of CHLH-ABAR, but upstream of the WRKY40 transcription factor. The other CPN20 interaction partners CPN10 and CPN60 are not involved in ABA signaling. Our findings show that CPN20 functions negatively in the ABAR-WRKY40 coupled ABA signaling independently of its co-chaperonin role, and provide a new insight into the role of co-chaperones in the regulation of plant responses to environmental cues.

KeywordsAbscisic acid signaling Arabidopsis thaliana Cochaperonin CPN20 Mg-chelatase H subunit WRKY40 transcription factor Xiao-Feng Zhang, Tao Jiang and Zhen Wu contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1007-s11103-013-0082-8 contains supplementary material, which is available to authorized users.

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Autor: Xiao-Feng Zhang - Tao Jiang - Zhen Wu - Shu-Yuan Du - Yong-Tao Yu - Shang-Chuan Jiang - Kai Lu - Xiu-Jing Feng - Xiao-Fang


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