Quantitative trait loci QTLs analysis of palm oil fatty acid composition in an interspecific pseudo-backcross from Elaeis oleifera H.B.K. Cortés and oil palm Elaeis guineensis Jacq.Reportar como inadecuado




Quantitative trait loci QTLs analysis of palm oil fatty acid composition in an interspecific pseudo-backcross from Elaeis oleifera H.B.K. Cortés and oil palm Elaeis guineensis Jacq. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Tree Genetics and Genomes

, Volume 9, Issue 5, pp 1207–1225

First Online: 21 June 2013Received: 23 August 2012Revised: 24 March 2013Accepted: 04 April 2013

Abstract

We chose an Elaeis interspecific pseudo-backcross of first generation E. oleifera × E. guineensis × E. guineensis to identify quantitative trait loci QTLs for fatty acid composition of palm oil. A dense microsatellite linkage map of 362 loci spanned 1.485 cM, representing the 16 pairs of homologous chromosomes in the Elaeis genus from which we traced segregating alleles from both E. oleifera and E. guineensis grandparents. The relative linear orders of mapped loci suggested the probable absence of chromosome rearrangements between the E. oleifera and E. guineensis genomes. A total of 19 QTL associated to the palm oil fatty acid composition were evidenced. The QTL positions and the species origin as well as the estimated effects of the QTL marker alleles were in coherence with the knowledge of the oil biosynthesis pathway in plants and with the individual phenotypic correlations between the traits. The mapping of chosen Elaeis key genes related to oleic acid C18:1, using intra-gene SNPs, supported several QTLs underlying notably FATA and SAD enzymes. The high number of hyper-variable SSR loci of known relative linear orders and the QTL information make these resources valuable for such mapping study in other Elaeis breeding materials.

KeywordsElaeis Oil palm E. oleifera QTL Fatty acid AbbreviationsACCaseAcetyl-CoA carboxylase

ACPAcyl carrier protein

AFLPAmplified fragment length polymorphism

ANOVAAnalysis of variance

C14:0Myristic acid

C16:0Palmitic acid

C16:1Palmitoleic acid C16:1

C18:0Stearic acid

C18:1Oleic acid C18:1

C18:2Linoleic acid C18:2

C18:3Linolenic acid C18:3

C20:0Arachidic acid

C20:1Gadoleic acid C20:1

EREndoplasmic reticulum

FATAAcyl-ACP thioesterase type A

FATBAcyl-ACP thioesterase type B

IMInterval mapping

IVIodine value

LGLinkage group

KAS I II, IIIβ-Ketoacyl-ACP synthase I, II, III

K-WKruskal–Wallis

MASMarker-assisted selection

MQMMultiple-QTL model

QTLQuantitative trait loci

RFLPRestriction fragment length polymorphism

SADΔ9 stearoyl-ACP desaturase

SNPSingle nucleotid polymorphism

SSHSuppression substractive hybridization

SSRSimple sequence repeat

TAGTriacylglycerol

Communicated by D. Grattapaglia

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Autor: Carmenza Montoya - Ricardo Lopes - Albert Flori - David Cros - Teresa Cuellar - Maryline Summo - Sandra Espeout - Ronan Ri

Fuente: https://link.springer.com/



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