In-vitro immunomodulatory and anti-cancerous activities of biotransformed products of Dianabol through Azadirachta indica and its molecular docking studiesReportar como inadecuado




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Chemistry Central Journal

, 7:163

Biological Chemistry

Abstract

BackgroundPlant Biotransformation is one of the tools for structural modifications of the organic substrate of low, moderate or high biological value utilizing plant cultured cells, these modifications of organic structures may lead to biologically augmented products and which may be ultimately substantial in cure or improvement of various morbidities and diseases.

ResultsAzadirachta indica A. Juss. suspension culture was employed for the biotransformation of dianabol 1 for the first time, and two metabolites, 17β-hydroxy-17α-methyl-5α-androst-1-en-3-one 2, and 17β-hydroxy-17α-methyl-5α-androstan-3-one 3 were obtained.

ConclusionsMost important aspect of this work was the evaluation of metabolite 2, which strongly and differentially suppressed not affecting whole blood and human polymorphonuclear cells PMN the phytohemagglutinin PHA-activated T-cell proliferation IC50: <10.33 μM, and also found to inhibit IL-2 production IC50: 16.89 ± 1.32 unlike metabolite 3 and compound 1. Compound 2 also exhibited anticancer activity against lung cancer cell line; NCI-H460, it moderately inhibited the growth of cancer cells 22.5 ± 4.15 μM. Furthermore, a good correlation between the predicted binding energies of the compounds acquired by the FlexX program and the experimental affinities were speculated upon interacting with IL-2 protein during molecular docking studies.

Open image in new windowKeywordsBiotransformation Anti-inflammatory activity Anti-cancer activity Molecular docking studies Electronic supplementary materialThe online version of this article doi:10.1186-1752-153X-7-163 contains supplementary material, which is available to authorized users.

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Autor: Saifullah - Saifullah Khan - Azizuddin - Sobia Ahsan Halim - Muhammad Kashif - Almas Jabeen - Muhmammad Asif - Muhammad Ahm

Fuente: https://link.springer.com/



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