Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host responseReportar como inadecuado




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Clinical Proteomics

, 10:14

First Online: 14 October 2013Received: 30 April 2013Accepted: 17 September 2013

Abstract

BackgroundChikungunya is a highly debilitating febrile illness caused by Chikungunya virus, a single-stranded RNA virus, which is transmitted by Aedes aegypti or Aedes albopictus mosquito species. The pathogenesis and host responses in individuals infected with the chikungunya virus are not well understood at the molecular level. We carried out proteomic profiling of serum samples from chikungunya patients in order to identify molecules associated with the host response to infection by this virus.

ResultsProteomic profiling of serum obtained from the infected individuals resulted in identification of 569 proteins. Of these, 63 proteins were found to be differentially expressed ≥ 2-fold in patient as compared to control sera. These differentially expressed proteins were involved in various processes such as lipid metabolism, immune response, transport, signal transduction and apoptosis.

ConclusionsThis is the first report providing a global proteomic profile of serum samples from individuals infected with the chikungunya virus. Our data provide an insight into the proteins that are involved as host response factors during an infection. These proteins include clusterin, apolipoproteins and S100A family of proteins.

KeywordsiTRAQ Quantitative proteomics Arthritis Hyponatremia Neuropathology AbbreviationsWHOWorld Health Organization

CDCCentre for Disease Control

iTRAQisobaric tag for Relative and Absolute Quantitation

PSMPeptide Spectral Match

PPDPlasma Proteome Database

HPRDHuman Protein Reference Database

GBSGuillain-Barré syndrome

FDRFalse Discovery Rate.

Electronic supplementary materialThe online version of this article doi:10.1186-1559-0275-10-14 contains supplementary material, which is available to authorized users.

Vinuth N Puttamallesh, Sreelakshmi K Sreenivasamurthy contributed equally to this work.

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Autor: Vinuth N Puttamallesh - Sreelakshmi K Sreenivasamurthy - Pradeep Kumar Singh - H C Harsha - Anjali Ganjiwale - Shobha Br

Fuente: https://link.springer.com/



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