Lack of in vitro effect of aglepristone on IFN-γ and IL-4 production by resting and mitogen-activated T cells of luteal bitchesReportar como inadecuado




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BMC Veterinary Research

, 9:220

Clinical pathology, physiology and immunology

Abstract

BackgroundAglepristone RU534 is an antiprogestin used for pregnancy termination, parturition induction and conservative pyometra treatment in bitches. Its molecular structure is similar to mifepristone, an antiprogestin used in human medicine. Mifepristone has been shown to suppress proliferation and cytokine production by T cells, whereas the effect of aglepristone on T cell function remains elusive. The purpose of this project was to investigate the in vitro influence of RU534 on IFN-γ and IL-4 synthesis by peripheral blood T cells isolated from healthy bitches N = 16 in luteal phase. The peripheral blood mononuclear cells PBMCs were incubated with three different dosages of aglepristone, or dimethyl sulfoxide DMSO, with or without mitogen. The production of cytokines by resting or mitogen-activated T cells was determined by intercellular staining and flow cytometry analysis or ELISA assay, respectively.

ResultsOur results showed no statistically significant differences in the percentage of IFN-γ and IL-4-synthesizing CD4 or CD8 resting T cells between untreated and aglepristone-treated cells at 24 and 48 hours post treatment. Moreover, mitogen-activated PBMCs treated with RU534 displayed similar concentration of IFN-γ and IL-4 in culture supernatants to those observed in mitogen-activated DMSO-treated PBMCs. Presented results indicate that administration of aglepristone for 48 hours has no influence on IFN-γ and IL-4 synthesis by resting and mitogen-activated T cells isolated from diestral bitches.

ConclusionsWe conclude that antiprogestins may differentially affect T cell function depending on the animal species in which they are applied.

KeywordsAglepristone Mifepristone Bitch T cells Cytokines Electronic supplementary materialThe online version of this article doi:10.1186-1746-6148-9-220 contains supplementary material, which is available to authorized users.

Piotr Jurka, Lidia Szulc-Dąbrowska, Joanna Borkowska contributed equally to this work.

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Autor: Piotr Jurka - Lidia Szulc-Dąbrowska - Joanna Borkowska - Anna Winnicka

Fuente: https://link.springer.com/







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