High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coliReportar como inadecuado

High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Microbial Cell Factories

, 12:97

First Online: 27 October 2013Received: 14 June 2013Accepted: 14 October 2013


BackgroundFor their application in the area of diagnosis and therapy, single-domain antibodies sdAbs offer multiple advantages over conventional antibodies and fragments thereof in terms of size, stability, solubility, immunogenicity, production costs as well as tumor uptake and blood clearance. Thus, sdAbs have been identified as valuable next-generation targeting moieties for molecular imaging and drug delivery in the past years. Since these probes are much less complex than conventional antibody fragments, bacterial expression represents a facile method in order to produce sdAbs in large amounts as soluble and functional proteins.

ResultsBy the combined use of high cell density cultivation media with a genetically engineered E. coli mutant strain designed for the cytoplasmic formation of proper disulfide bonds, we achieved high level of intracellular sdAb production up to 200 mg-L. Due to a carboxyterminal hexahistidine epitope, the soluble recombinant sdAbs could be purified by one-step immobilized metal affinity chromatography to apparent homogeneity and easily radiolabeled with Tc within 1 h. The intradomain disulfide bridge being critical for the stability and functionality of the sdAb molecule was shown to be properly formed in ~96% of the purified proteins. In vitro binding studies confirmed the high affinity and specificity of the expressed sdAb 7C12 towards its molecular target.

ConclusionsOur study demonstrates an efficient cultivation and expression strategy for the production of substantial amounts of soluble and functional sdAbs, which may be adopted for high-yield production of other more complex proteins with multiple disulfides as well.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2859-12-97 contains supplementary material, which is available to authorized users.

Download fulltext PDF

Autor: Kristof Zarschler - Stefanie Witecy - Franz Kapplusch - Christian Foerster - Holger Stephan

Fuente: https://link.springer.com/

Documentos relacionados