Degradation of lipoxygenase-derived oxylipins by glyoxysomes from sunflower and cucumber cotyledonsReportar como inadecuado




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BMC Plant Biology

, 13:177

Biochemistry and physiology

Abstract

BackgroundOilseed germination is characterized by the degradation of storage lipids. It may proceed either via the direct action of a triacylglycerol lipase, or in certain plant species via a specific lipid body 13-lipoxygenase. For the involvement of a lipoxygenase previous results suggested that the hydroxy- or oxo-group that is being introduced into the fatty acid backbone by this lipoxygenase forms a barrier to continuous β-oxidation.

ResultsThis study shows however that a complete degradation of oxygenated fatty acids is possible by isolated cucumber and sunflower glyoxysomes. Interestingly, degradation is accompanied by the formation of saturated short chain acyl-CoAs with chain length between 4 and 12 carbon atoms lacking the hydroxy- or oxo-diene system of the oxygenated fatty acid substrate. The presence of these CoA esters suggests the involvement of a specific reduction of the diene system at a chain length of 12 carbon atoms including conversion of the hydroxy-group at C7.

ConclusionsTo our knowledge this metabolic pathway has not been described for the degradation of polyunsaturated fatty acids so far. It may represent a new principle to degrade oxygenated fatty acid derivatives formed by lipoxygenases or chemical oxidation initiated by reactive oxygen species.

KeywordsCoA-ester Electrophiles Lipid oxidation Lipoxygenase pathway β-oxidation AbbreviationsCoACoenzyme A

HPODHydroperoxy octadecadienoic acid

LALinoleic acid

KODKeto octadecadienoic acid

LOXLipoxygenase

MSMass spectrometry

NADNicotinamide adenine dinucleotide

RTRetention time

TEATriethylamine

TAGTriacylglycerol.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2229-13-177 contains supplementary material, which is available to authorized users.

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Autor: Danilo Meyer - Cornelia Herrfurth - Florian Brodhun - Ivo Feussner

Fuente: https://link.springer.com/







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