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BMC Complementary and Alternative Medicine

, 13:321

Basic research

Abstract

BackgroundExtracts from Potentilla species have been applied in traditional medicine and exhibit antioxidant, hypoglycemic, anti-inflammatory, antitumor and anti-ulcerogenic properties, but little has been known about the diversity of phytochemistry and pharmacology on this genus. This study investigated and compared the phytochemical profiles, antioxidant and antimicrobial activities of leaf extracts from three Potentilla species Potentilla fruticosa, Potentilla glabra and Potentilla parvifolia in order to discover new resources for lead structures and pharmaceutical products.

MethodsChemical composition and content of six phenolic compounds were evaluated and determined by RP-HPLC; Total phenolic and total flavonoid content were determined using Folin-Ciocalteau colourimetric method and sodium borohydride-chloranil-based method SBC; Antioxidant activities were determined using DPPH, ABTS and FRAP assays; Antimicrobial properties were investigated by agar dilution and mycelial growth rate method.

ResultsThe results showed hyperoside was the predominant phenolic compound in three Potentilla species by RP-HPLC assay, with the content of 8.86 P. fruticosa, 2.56 P. glabra and 2.68 mg-g P. parvifolia, respectively. The highest content of total identified phenolic compounds hyperoside, +-catechin, caffeic acid, ferulic acid, rutin and ellagic acid was observed in P. parvifolia 14.17 mg-g, follow by P. fruticosa 10.01 mg-g and P. glabra 7.01 mg-g. P. fruticosa possessed the highest content of total phenolic 84.93 ± 0.50 mmol gallic acid equivalent-100 g and total flavonoid 84.14 ± 0.03 mmol quercetin equivalent-100 g, which were in good correlation with its significant DPPHIC50 16.87 μg-mL, ABTS 2763.48 μmol Trolox equivalent-g and FRAP 1398.70 μmol Trolox equivalent-g capacities. Furthermore, the effective methodology to distinguish the different species of Potentilla was also established by chromatographic fingerprint analysis for the first time. The results of antimicrobial activities showed P. fruticosa exhibited the strongest inhibition aganist Gram-positive bacteria, Pseudomonas aeruginosa and Candida albicans with MIC values of 0.78–6.25 mg-mL. P. parvifolia possessed antibacterial and antifungal activities against all the microorganisms tested, with EC50 and MIC values of 20.52–47.02 mg-mL and 0.78–50 mg-mL, respectively.

ConclusionsThese results indicated that leaf extracts from three Potentilla species could become useful supplement for pharmaceutical products as a new antioxidant and antimicrobial agents.

KeywordsPotentilla spp Phytochemicals Antioxidant activity Antimicrobial activity RP-HPLC Electronic supplementary materialThe online version of this article doi:10.1186-1472-6882-13-321 contains supplementary material, which is available to authorized users.

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Autor: Shan-Shan Wang - Dong-Mei Wang - Wen-Jun Pu - Deng-Wu Li

Fuente: https://link.springer.com/







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