In vitro bone inducing effects of Lentinula edodes shiitake water extract on human osteoblastic cell culturesReportar como inadecuado




In vitro bone inducing effects of Lentinula edodes shiitake water extract on human osteoblastic cell cultures - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Natural Products and Bioprospecting

, Volume 3, Issue 6, pp 282–287

First Online: 03 December 2013Received: 13 September 2013Accepted: 16 November 2013

Abstract

The effect of Lentinula edodes water extract LE on two osteoblastic cell cultures HOS 58 and Saos-2 was investigated to determine if this edible medicinal mushroom has osteoinductive properties. Activity of alkaline phosphatase and mineralization were used as indicators for the vitality and maturation of the bone cells. Cultivation of human osteosarcoma cells HOS 58 for five days in presence of a serial dilution of the aqueous extract of L. edodes 0.8 μg-mL-125 μg-mL resulted in a significant elevation of alkaline phosphatase activity ALP of the cells in comparison to untreated cells. Saos-2 cells, incubated with LE 20 μg-mL and β-glycerol phosphate 2 mM for 21 days, displayed a 2 fold level of mineralization than cells cultured soley with the positive control, β-glycerophosphate. The obtained results clearly indicate the activity of LE as a bone inducing agent in vitro. Therefore, the shiitake mushroom L. edodes deserves attention as a supportive dietary treatment or nutraceutical in the case of diseases accompanied with bone disorder, such as osteoporosis, osteopenia, and late complication of diabetes. Open image in new windowKeywordsmedicinal mushrooms Lentinula edodes osteoporosis alkaline phosphatase bone mineralization shiitake This article is published with open access at Springerlink.com

Download to read the full article text



Autor: Ashraf Saif - Kristian Wende - Ulrike Lindequist

Fuente: https://link.springer.com/



DESCARGAR PDF




Documentos relacionados