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Plant Molecular Biology Reporter

, Volume 31, Issue 6, pp 1529–1538

First Online: 25 July 2013

Abstract

Production of recombinant proteins in plants is of increasing importance for practical applications. However, the production of stable transformed transgenic plants is a lengthy procedure. Transient expression, on the other hand, can deliver recombinant proteins within a week, and many viral vectors have been constructed for that purpose. Each of them is reported to be highly efficient, robust and cost-effective. Here, a variety of expression vectors which were designed for transient and stable plant transformation, including pPZP3425, pPZP5025, pPZPTRBO, pJLTRBO, pEAQ-HT and pBY030-2R, was compared for the expression of green fluorescent protein and β-glucuronidase in Nicotiana benthamiana by Agrobacterium-mediated transient expression. Our results show that pPZPTRBO, pJLTRBO and pEAQ-HT had comparable expression levels without co-infiltration of a RNA-silencing inhibitor. The other vectors, including the non-viral vectors pPZP5025 and pPZP3425, needed co-infiltration of the RNA-silencing inhibitor P19 to give good expression levels.

KeywordsTransient expression Recombinant protein Agroinfiltration Viral vector pPZP vector family AbbreviationsCaMVCauliflower mosaic virus

dpiDays post infiltration

HRHypersensitive response

4-MU4-Methylumbelliferone

4-MUG4-Methylumbelliferyl-β-d-glucuronide

TBSVTomato bushy stunt virus

TMVTobacco mosaic virus

TSPTotal soluble protein

Electronic supplementary materialThe online version of this article doi:10.1007-s11105-013-0614-z contains supplementary material, which is available to authorized users.

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Autor: Kausar Hussain Shah - Bachar Almaghrabi - Holger Bohlmann

Fuente: https://link.springer.com/



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