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Microbial Cell Factories

, 12:124

Added Value Chemicals and Biofuels

Abstract

BackgroundBacillus subtilis 168 possesses an efficient pathway to metabolize some of the stereoisomers of inositol, including myo-inositol MI and scyllo-inositol SI. Previously we reported a prototype of a B. subtilis cell factory with modified inositol metabolism that converts MI into SI in the culture medium. However, it wasted half of initial 1.0% w-v MI, and the conversion was limited to produce only 0.4% w-v SI. To achieve a more efficient SI production, we attempted additional modifications.

ResultsAll -useless- genes involved in MI and SI metabolism were deleted. Although no elevation in SI production was observed in the deletion strain, it did result in no wastage of MI anymore. Thus additionally, overexpression of the key enzymes, IolG and IolW, was appended to demonstrate that simultaneous overexpression of them enabled complete conversion of all MI into SI.

ConclusionsThe B. subtilis cell factory was improved to yield an SI production rate of 10 g-L-48 h at least. The improved conversion was achieved only in the presence of enriched nutrition in the form of 2% w-v Bacto soytone in the medium, which may be due to the increasing demand for regeneration of cofactors.

KeywordsBacillus subtilis scyllo-inositol myo-inositol Bioconversion Alzheimer’s disease AbbreviationsMImyo-inositol

SIscyllo-inositol

AβAmyloid-beta.

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2859-12-124 contains supplementary material, which is available to authorized users.

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Autor: Kosei Tanaka - Shintaro Tajima - Shinji Takenaka - Ken-ichi Yoshida

Fuente: https://link.springer.com/







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