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Gut Pathogens

, 5:38

First Online: 11 December 2013Received: 30 October 2013Accepted: 18 November 2013

Abstract

BackgroundBacteria exist widely in a diversity of natural environments. In order to survive adverse conditions such as nutrient depletion, biochemical and biological disturbances, and high temperature, bacteria have developed a wide variety of coping mechanisms. Temperature is one of the most important factors that can enhance the expression of microbial proteins. This study was conducted to investigate how outer membrane proteins OMPs of the bacterium Shigella flexneri respond to stress, especially during fever when the host’s body temperature is elevated.

MethodsOMPs of S. flexneri ATCC 12022 and clinical isolate SH057 were extracted from an overnight culture grown at 37, 38.5, and 40°C. Comparisons of the expressed proteins under the different growth conditions were based on equal numbers of bacterial cells loaded in the SDS-PAGE gels. Separated proteins were stained with Coomassie brilliant blue. Selected proteins showing increased expression at 38.5 and 40°C were characterized by performing MALDI-ToF-ToF.

ResultsDifferent degrees of expression were demonstrated for different proteins expressed at 37°C compared to 38.5 and 40°C. The proteins with molecular sizes of 18.4, 25.6, and 57.0 kDa showed increased expression level at increasing temperature and were identified as Dps, WrbA, and PepA, respectively.

ConclusionThis study revealed that strains of S. flexneri respond at the proteomic level during stress caused by elevated temperature by decreasing the expression of proteins, maintaining the level of important proteins, or enhancing the levels of proteins presumably involved in survival and virulence.

Electronic supplementary materialThe online version of this article doi:10.1186-1757-4749-5-38 contains supplementary material, which is available to authorized users.

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Autor: Hemavathy Harikrishnan - Asma Ismail - Kirnpal-Kaur Banga Singh

Fuente: https://link.springer.com/



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