Ascorbic acid enhances adipogenesis of 3T3-L1 murine preadipocyte through differential expression of collagensReportar como inadecuado

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Lipids in Health and Disease

, 12:182

First Online: 11 December 2013Received: 16 September 2013Accepted: 28 November 2013


BackgroundAdipogenesis from preadipocytes into mature adipocyte is precisely coordinated by transcription factors such as CCAAT-enhancer-binding proteins C-EBPs and peroxisome proliferator-activated receptor γ PPARγ, cytokines, and hormones, which is accompanied by extracellular matrix remodeling. Besides anti-oxidant activity, ascorbic acid ASC is participating in collagen biosynthesis and increase production and processing of collagens. Moreover, several studies demonstrated that ASC enhanced differentiation from preadipocytes into mature adipocytes.

MethodsThe adipogenic effect of ascorbic acid was evaluated in chemical induced 3T3-L1 by Oil Red O staining. This effect was elucidated by immunoblotting which detected the expression level of collagens and transcription factors in adipogenesis. The immunocytochemical determination of type I collagen was performed in 3T3-L1 adipocyte to show the change of extracellular matrix during adipogenesis.

ResultsIn this study, Oil Red O staining in 3T3-L1 preadipocytes was increased dose-dependently by addition of ASC. These ASC-treated adipocytes increased collagen processing of α1I and α1V and expressed α1VI and α2VI collagens differentially. ASC also stimulated expression of C-EBPα and PPARγ, which is preceded by collagen enhancement. In addition, inhibition of ASC activity by ethyl-3,4-dihydroxybenzoate showed reduction of lipid accumulation by removal of large lipid droplets, not by inhibition of lipid production. This observation went with loss of α1I deposition on adipocyte surface, increase of α1V and α2VI collagens and decrease of C-EBPs.

ConclusionOur findings imply that various actions of ASC on adipogenesis through differential collagen expression may provide diverse applications of ASC to adipose tissue technology.

KeywordsAscorbic acid adipogenesis 3T3-L1 collagens differential expression AbbreviationsASCAscorbic acid

C-EBPsCCAAT-enhancer-binding proteins


PPARγperoxisome proliferator-activated receptor γ

OROOil Red O.

Electronic supplementary materialThe online version of this article doi:10.1186-1476-511X-12-182 contains supplementary material, which is available to authorized users.

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Autor: Byoungjae Kim - Kyung Min Choi - Hong Soon Yim - Min-Goo Lee


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