Preliminary antimycobacterial study on selected Turkish plants Lamiaceae against Mycobacterium tuberculosis and search for some phenolic constituentsReportar como inadecuado

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BMC Complementary and Alternative Medicine

, 13:365

Basic research


BackgroundThe global resurgence of tuberculosis is a significant threat. Lamiaceae members have been used in folk remedies for centuries. This study was designed to assess the in-vitro antimycobacterial activity of eighteen crude extracts from six plants Lamiaceae and to characterize their phenolic and flavonoid compounds.

MethodsSix Turkish medicinal plants of the family Lamiaceae Stachys tmolea Boiss., Stachys thirkei C. Koch, Ballota acetabulosa L. Benth., Thymus sipthorpii Benth., Satureja aintabensis P.H. Davis, and Micromeria juliana L. Benth. ex Reich. were collected in 2009 – 2010. Dried and crushed plant samples were subjected to sequential extraction with petroleum ether, ethyl acetate, and methanol in order of increasing polarity. A broth microdilution method was employed to screen extracts against four mycobacterial strains of Mycobacterium tuberculosis. Phenolic and flavonoid compounds were characterized by liquid chromatography–mass spectrometry.

ResultsS. aintabensis, T. sibthorpii, and M. juliana were found to develop considerable activity against the four strains of M. tuberculosis with the minimal inhibitory concentrations value of 12.5-100 μg-ml. S. aintabensis and T. sibthorpii extracts killed M. tuberculosis with the minimum bactericidal concentration value of 50–800 μg-ml. On the basis of these prominent antimycobacterial activity, we suggest that they could be a source of natural anti-tuberculosis agents.

ConclusionS. aintabensis and T. sibthorpii showed activity by killing Mycobacteria strains. The major phenolic compound was rosmarinic for T. sibthorpii and S. aintabensis. Flavonoids might be -a modal- for the drug design.

KeywordsMycobacterium tuberculosis Antimycobacterial activity Satureja aintabensis Thymus sipthorpii, Lamiaceae Phenolic Flavonoid AbbreviationsTBTuberculosis

MDRMulti-drug resistance

SLDSecond-line drug

DOTSDirectly observed treatment short course

HIVHuman immunodeficiency virus

RtRetention time

ATCCAmerican type culture collection

OADCOleic acid albumin, dextrose, and catalase

PANTAPolymixin amphotericin B, naladixic acid, trimethoprim and azlocillin

MGITMycobacteria growth indicator tube

BACTECBattle area clearance training, equipment and consultancy






ESIElectrospray ionisation


PEFPetroleum ether fraction

EAFEthyl acetate fraction

MEFMethanol fraction

ASTAntimicrobial susceptibility testing

MICMinimal inhibitory concentration

MBCMinimal bactericide concentration

LC-MSLiquid chromatography–mass spectrometry

MPBAMicroplate prestoblue alamar assay

ESIElectrospray ionisation

TICTotal ion current

SIMSelected ion monitoring.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6882-13-365 contains supplementary material, which is available to authorized users.

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Autor: Tülin Askun - Emmanuel Mouafo Tekwu - Fatih Satil - Seyma Modanlioglu - Hatice Aydeniz


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