Surface-enhanced Raman scattering SERS revealing chemical variation during biofilm formation: from initial attachment to mature biofilmReportar como inadecuado




Surface-enhanced Raman scattering SERS revealing chemical variation during biofilm formation: from initial attachment to mature biofilm - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Analytical and Bioanalytical Chemistry

, Volume 404, Issue 5, pp 1465–1475

First Online: 21 July 2012Received: 29 March 2012Revised: 20 June 2012Accepted: 21 June 2012

Abstract

Surface-enhanced Raman scattering SERS has recently been proved to be a promising technique for characterizing the chemical composition of the biofilm matrix. In the present study, to fully understand the chemical variations during biofilm formation, SERS based on silver colloidal nanoparticles was applied to evaluate the chemical components in the matrix of biofilm at different growth phases, including initial attached bacteria, colonies, and mature biofilm. Meanwhile, atomic force microscopy was also applied to study the changes of biofilm morphology. Three model bacteria, including Escherichia coli, Pseudomonas putida, and Bacillus subtilis, were used to cultivate biofilms. The results showed that the content of carbohydrates, proteins, and nucleic acids in the biofilm matrix increased significantly along with the biofilm growth of the three bacteria judging from the intensities and appearance probabilities of related marker peaks in the SERS spectra. The content of lipids, however, only increased in the Gram-negative biofilms E. coli and P. putida rather than the Gram-positive biofilm B. subtilis. Our findings strongly suggest the SERS has significant potential for studying chemical variations during biofilm formation.

Open image in new windowFigureAchieving surface-enhanced Raman scattering by coating silver nanoparticles on biofilm surface.

KeywordsSERS Biofilm formation Biofilm matrix Raman microscopy Atomic force microscopy  Download fulltext PDF



Autor: Yuanqing Chao - Tong Zhang

Fuente: https://link.springer.com/







Documentos relacionados