Identification of B cell epitopes reactive to human papillomavirus type-16L1- derived peptidesReport as inadecuate

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Virology Journal

, 9:199

First Online: 14 September 2012Received: 22 February 2012Accepted: 11 September 2012


BackgroundPersistent infection of human papillomavirus HPV types 16 and 18 causes cervical cancer. To better understand immune responses to the prophylactic vaccine, HPV 16-18 L1 virus-like particles HPV-VLPs, we investigated B cell epitopes of HPV16 L1-derived peptides.

MethodsSera from mice immunized with HPV-16-18 L1 VLPs were analyzed for their IgG titers against 10 different HPV16 L1-derived peptides 20-mer that contain human leukocyte antigen HLA-class I A-2, A-24 and class II DR.

ResultsOne 20-mer peptide at positions 300 to 319 was identified as a common B cell epitope in both Balb-c H-2 and C57BL-6 H-2 mice. Mapping analysis showed that the 10-amino-acid sequence at positions 304to 313 was an immunogenic portion. It is of note that the binding capability of this 10-mer peptide to the HLA-A2 and HLA-A24 molecules was confirmed by the HLA class I stabilization assay. In addition, one unique 20-mer was determined as a B cell epitope in each strain.

ConclusionsThese results might provide new information for better understanding of immune responses to HPV 16 L1.

KeywordsHuman papillomavirus Prophylactic vaccine Anti-peptide antibody Virus-like particles AbbreviationsHPVHuman papillomavirus

VLPsVirus-like particles

HLAHuman leukocyte antigen

FIUFluorescent intensity units.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-9-199 contains supplementary material, which is available to authorized users.

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Author: Akimasa Fukui - Satoko Matsueda - Kouichiro Kawano - Naotake Tsuda - Nobukazu Komatsu - Shigeki Shichijo - Tetsuro Sasada -


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