Comparative analysis of hemolymph proteome maps in diapausing and non-diapausing larvae of Sesamia nonagrioides - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Proteome Science

, 10:58

First Online: 28 September 2012Received: 26 May 2012Accepted: 24 September 2012

Abstract

BackgroundSesamia nonagrioides is a noctuid that feeds on maize, sugar cane and sorghum in North Africa and Southern Europe. Larvae reared under long day conditions pupate after 5 or 6 larval instars, whereas larvae reared under short day conditions enter diapause and undergo up to 12 molts before dying or pupating. To better understand the mechanism of larval development and diapause, we identified proteins with different expressions in the sixth instar of diapausing and non-diapausing larvae.

ResultsA total of 52 differentially regulated proteins were detected in the hemolymph of the diapausing or non-diapausing larvae at the beginning or end of the sixth instar. From these proteins, 11 were identified by mass spectrometry MALDI-TOF MS or MALDI-TOF-TOF MS-MS: 5 were upregulated in the hemolymph of non-diapausing larvae and 6 in the hemolymph of the diapausing larvae. Interestingly, some proteins were expressed only in non-diapausing larvae but none was expressed only in the hemolymph of diapausing larvae. The possible functions of some of these proteins related to diapause maintenance or to larval-pupal metamorphosis are discussed.

ConclusionsThe 2-DE proteomic map of S. nonagrioides hemolymph shows differential protein expression in diapausing and non-diapausing larvae. Some proteins that showed higher expression in the diapausing larvae at the end of the sixth instar could be involved in JH level maintenance thus in the diapause status maintenance. On the contrary, other proteins that showed the highest expression or that were expressed only in the non-diapausing larvae could be involved in larval-pupal metamorphosis.

KeywordsDiapause Proteomic analysis Hemolymph 2D electrophoresis Mass spectrometry Sesamia nonagrioides Abbreviations2-DE gelTwo- dimensional electrophoresis gel

JHJuvenile hormone

L6d2Larvae of the sixth instar two days old

L6d7Larvae of the sixth instar seven days old

LDLong day photoperiod

SDShort day photoperiod

pIIsoelectric point

MS-MSTandem mass spectrometry

FATPFatty acid transport protein

Gyc-89 DaSoluble guanylate cyclase 89 Da

cGMPCyclicguanosine monophosphate

JHBPJuvenile hormone binding protein

ATPAdenosine triphosphate

hsp7070 kilodalton heat shock proteins

DGKDiacylglycerol kinase

MocoMolybdenum cofactor

PKCProtein kinase C1

CHR3Caenorhabditis elegans orphan nuclear hormone receptor

2D-PAGETwo- dimensional polyacrylamide gel electrophoresis

IEFIsoelectric focusing

LOESSNew procedure in SAS-STAT software for performing local regression

MALDI-TOF-TOFMatrix-assisted laser desorption-ionization time of flight

LIFTLaser-induced forward transfer

NCBINational center for biotechnology information

BLASTBasic local alignment search tool.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-5956-10-58 contains supplementary material, which is available to authorized users.

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Autor: Meritxell Pérez-Hedo - Isabel Sánchez-López - Matilde Eizaguirre

Fuente: https://link.springer.com/

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