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Optical Nanoscopy

, 1:7

First Online: 28 September 2012Received: 12 July 2012Accepted: 11 September 2012


In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion STED microscopy, providing < 25 nm spatial resolution in raw images of cells.

KeywordsMicroscopy Confocal STED Fluorescence Rhodamines Bioconjugation Super-resolution Nanoscopy Stimulated emission depletion Electronic supplementary materialThe online version of this article doi:10.1186-2192-2853-1-7 contains supplementary material, which is available to authorized users.

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Autor: Christian A Wurm - Kirill Kolmakov - Fabian Göttfert - Haisen Ta - Mariano Bossi - Heiko Schill - Sebastian Berning - Ste


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