Molecular Modeling of Metabolism for Allergen-Free Low Linoleic Acid PeanutsReport as inadecuate




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Applied Biochemistry and Biotechnology

, Volume 168, Issue 4, pp 805–823

First Online: 24 August 2012Received: 27 January 2012Accepted: 01 August 2012

Abstract

It is necessary to eliminate linoleic acid and allergenic arachins from peanuts for good health reasons. Virginia-type peanuts, harvested from plots treated with mineral salts combinations that mimic the subunit compositions of glutamate dehydrogenase GDH were analyzed for fatty acid and arachin compositions by HPLC and polyacrylamide gel electrophoresis, respectively. Fatty acid desaturase and arachin encoding mRNAs were analyzed by Northern hybridization using the homologous RNAs synthesized by peanut GDH as probes. There were 70–80 % sequence similarities between the GDH-synthesized RNAs and the mRNAs encoding arachins, fatty acid desaturases, glutamate synthase, and nitrate reductase, which similarities induced permutation of the metabolic pathways at the mRNA level. Modeling of mRNAs showed there were 210, 3,150, 1,260, 2,520, and 4,200 metabolic permutations in the control, NPKS-, NS-, Pi-, NH4Cl-, and PK-treated peanuts, respectively. The mRNA cross-talks decreased the arachin to almost zero percent in the NPKS- and PK-treated peanuts, and linoleate to ∼18 % in the PK-treated peanut. The mRNA cross-talks may account for the vastly reported environmentally induced variability in the linoleate contents of peanut genotypes. These results have quantitatively unified molecular biology and metabolic pathways into one simple biotechnology for optimizing peanut quality and may encourage small-scale industry to produce arachin-free low linoleate peanuts.

KeywordsGlutamate dehydrogenase-synthesized RNA Partial downregulation HPLC Northern hybridization mRNA permutation  Download fulltext PDF



Author: Godson O. Osuji - Tassine K. Brown - Sanique M. South - Dwiesha Johnson - Shanique Hyllam

Source: https://link.springer.com/







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