Covalently tethered transforming growth factor beta in PEG hydrogels promotes chondrogenic differentiation of encapsulated human mesenchymal stem cellsReport as inadecuate




Covalently tethered transforming growth factor beta in PEG hydrogels promotes chondrogenic differentiation of encapsulated human mesenchymal stem cells - Download this document for free, or read online. Document in PDF available to download.

Drug Delivery and Translational Research

, Volume 2, Issue 5, pp 305–312

First Online: 18 September 2012

Abstract

Methods to precisely control growth factor presentation in a local and sustained fashion are of increasing interest for a number of complex tissue engineering applications. The cytokine transforming growth factor beta TGFβ plays a key role in promoting the chondrogenic differentiation of human mesenchymal stem cells hMSCs. Traditional chondrogenic approaches utilize soluble delivery, an approach with limited application for clinical translation. In this work, we introduce a reactive thiol onto TGFβ and covalently tether the growth factor into polyethylene glycol PEG hydrogels using a photoinitiated thiol-acrylate polymerization mechanism. We demonstrate the bioactivity of thiolated TGFβ, before and after polymerization, using a SMAD2 reporter cell line. hMSCs were encapsulated in PEG hydrogels with and without tethered TGFβ, and subsequently assayed for glycosaminoglycan and collagen II production as indicators of chondrogenesis. Over a 21-day time course, tethered TGFβ promoted chondrogenesis at levels similar to a positive control using solubly dosed growth factor. These results provide evidence that tethered TGFβ materials can be successfully used to promote chondrogenic differentiation of MSCs.

KeywordsImmobilized growth factors Hydrogels Chondrogenesis Stem cell differentiation Photopolymerization Cell scaffold  Download fulltext PDF



Author: Joshua D. McCall - Jacob E. Luoma - Kristi S. Anseth

Source: https://link.springer.com/







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