An inexpensive antigen for serodiagnosis of chagas’ disease. Report as inadecuate




An inexpensive antigen for serodiagnosis of chagas’ disease. - Download this document for free, or read online. Document in PDF available to download.

Eva Mary Rodríguez ; Mehudy Medina ; Yelitza Campos ; Walter Mosca ; Amanda Briceño ; Graciela León ;Investigación Clínica 2010, 51 1

Author: Luis Briceño

Source: http://www.redalyc.org/articulo.oa?id=372937679010


Teaser



Investigación Clínica ISSN: 0535-5133 riclinicas@gmail.com Universidad del Zulia Venezuela Briceño, Luis; Rodríguez, Eva Mary; Medina, Mehudy; Campos, Yelitza; Mosca, Walter; Briceño, Amanda; León, Graciela An inexpensive antigen for serodiagnosis of Chagas’ disease. Investigación Clínica, vol.
51, núm.
1, 2010, pp.
103-113 Universidad del Zulia Maracaibo, Venezuela Available in: http:--www.redalyc.org-articulo.oa?id=372937679010 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative Invest Clin 51(1): 101 - 113, 2010 An inexpensive antigen for serodiagnosis of Chagas’ disease. Luis Briceño1, Eva Mary Rodríguez2, Mehudy Medina3, Yelitza Campos1, Walter Mosca1, Amanda Briceño4 and Graciela León5. Laboratory of Physiopathology, Institute of Biomedicine, Faculty of Medicine, Central University of Venezuela, 2Research Center in Public Health “Jacinto Convit”, Faculty of Medicine, Central University of Venezuela, Direccion General de Salud Ambiental, 3Ministerio de Salud, 4Universita degli Studi di Milano, Italia and 5Municipal Blood Bank, Caracas, Venezuela. 1 Key words: Chagas’disease, diagnostic, antigen, inexpensive. Abstract.
In this prospective study we evaluated the performance characteristics of a specific and sensitive antigen preparation (AgA) used in an enzyme-linked immunosorbent assay (ELISA) for the detection of antiTrypanosoma cruzi antibodies in serum samples, for Chagas’ disease diagnosis.
The antigen production was achieved by combination of nutritional stress and autoclaving the parasites.
Specificity and sensitivity were evaluated in two separate tests, using 152 sera from healthy individuals and 175 sera from Chagas’ patients (70 by xenodiagnosis).
Cross-reactivity was tested using 289 sera...





Related documents