Frequency of caseous lymphadenitis CLA in sheep slaughtered in an abattoir in Tabriz: comparison of bacterial culture and pathological studyReport as inadecuate

Frequency of caseous lymphadenitis CLA in sheep slaughtered in an abattoir in Tabriz: comparison of bacterial culture and pathological study - Download this document for free, or read online. Document in PDF available to download.

Comparative Clinical Pathology

, Volume 21, Issue 5, pp 667–671

First Online: 06 January 2011Received: 08 July 2010Accepted: 07 December 2010


From January to February 2008, 468 sheep carcasses 335 male and 133 female in a Khosroshahr suburb of Tabriz, East Azerbaijan province, Iran abattoir were randomly selected for inspection. The aim of the study was to estimate the frequency of caseous lymphadenitis CLA in sheep and to compare the results of bacterial cultures and histopathology of suspected cases. The mean age of the population was 2.5 years. One hundred ninety-seven cases containing 153 77.7% males and 44 22.3% females had prominent enlargement of one of the lymph nodes i.e., prescapular, prefemoral, inguinal, supramammary, or midiastinal; these were removed with the surrounding tissue for further evaluation. For confirmed diagnosis of CLA, samples were sent for microbiology and pathology analysis. Standard bacteriological culture methods for isolation of Corynebacterium pseudotuberculosis and tissue preparations for histopathological sections were performed. To evaluate the effect of age on the frequency of CLA, animals were categorized in four groups: under 1, 1–2, 2–3, and over 3 years of age. Based on the results, in 59 12.60% carcasses C. pseudotuberculosis was isolated, and in 94 20.08% of the cases histopathological studies revealed pathognomonic signs lamellated exudates or onion ring of CLA. The frequency of CLA based on bacteriological culture was 12.60% and on histopathological study 20.08%. In 37 18.8% of the carcasses, both bacteriological and histopathological studies confirmed CLA. The frequency of CLA following microscopic examination 20.08% presented a more precise diagnosis compared to bacteriological culture 12.60% and macroscopic evaluation of the lymph nodes P < 0.05. Furthermore, there was a positive correlation rate between the bacteriological culture and histopathological study r = 0.196, P = 0.006. The prescapular lymph node had the highest infection rate with 54 1.70 ± 0.97 and supramammary lymph node had the lowest with two 0.07 ± 0.41 P < 0.05. There was an increase in CLA detection with increasing age P < 0.05, the mean age of animals with a positive CLA test were 2.92 years and in the oldest age group 31 47% cases had the highest infection.

KeywordsCaseous lymphadenitis Sheep Frequency Tabriz Iran  Download fulltext PDF

Author: Fereydon Rezazadeh Zavoshti - Amir Babak Sioofy Khoojine - Javad Ashrafi Helan - Belal Hassanzadeh - Ali Akbar Heydari


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