Chromosome-wide regulation of euchromatin-specific 5mC to 5hmC conversion in mouse ES cells and female human somatic cellsReport as inadecuate




Chromosome-wide regulation of euchromatin-specific 5mC to 5hmC conversion in mouse ES cells and female human somatic cells - Download this document for free, or read online. Document in PDF available to download.

Chromosome Research

, Volume 20, Issue 7, pp 837–848

First Online: 31 October 2012Received: 10 August 2012Revised: 09 October 2012Accepted: 10 October 2012

Abstract

DNA cytosine methylation 5mC is indispensable for a number of cellular processes, including retrotransposon silencing, genomic imprinting, and X chromosome inactivation in mammalian development. Recent studies have focused on 5-hydroxymethylcytosine 5hmC, a new epigenetic mark or intermediate in the DNA demethylation pathway. However, 5hmC itself has no role in pluripotency maintenance in mouse embryonic stem cells ESCs lacking Dnmt1, 3a, and 3b. Here, we demonstrated that 5hmC accumulated on euchromatic chromosomal bands that were marked with di- and tri-methylated histone H3 at lysine 4 H3K4me2-3 in mouse ESCs. By contrast, heterochromatin enriched with H3K9me3, including mouse chromosomal G-bands, pericentric repeats, human satellite 2 and 3, and inactive X chromosomes, was not enriched with 5hmC. Therefore, enzymes that hydroxylate the methyl group of 5mC belonging to the Tet family might be excluded from inactive chromatin, which may restrict 5mC to 5hmC conversion in euchromatin to prevent nonselective de novo DNA methylation.

KeywordsDNA methylation Embryonic stem cells Tet enzymes 5-Hydroxymethylation Abbreviations5hmC5-Hydroxymethylcytosine

5mCDNA cytosine methylation

DnmtDNA methyltransferase

ESCsEmbryonic stem cells

iPSCsInduced pluripotential stem cells

MBDMethyl-binding domain

PGCsPrimordial germ cells

PNPronucleus

TetTet Ten-eleven translocation methylcytosine dioxygenase

Responsible Editor: Tatsuo Fukagawa.

Electronic supplementary materialThe online version of this article doi:10.1007-s10577-012-9317-9 contains supplementary material, which is available to authorized users.

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Author: Musashi Kubiura - Masaki Okano - Hiroshi Kimura - Fumihiko Kawamura - Masako Tada

Source: https://link.springer.com/







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