Cytokine response in patients with chronic infections caused by Staphylococcus aureus strains and diversification of their Agr system classesReport as inadecuate




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European Journal of Clinical Microbiology and Infectious Diseases

, Volume 31, Issue 10, pp 2809–2815

First Online: 26 May 2012Received: 15 March 2012Accepted: 20 April 2012

Abstract

This study aimed to describe the levels of circulating cytokine levels produced by Th lymphocytes IFN-γ, IL-4, IL-10, IL-17A, as well as the levels of cytokines produced by monocytes-macrophages TNF-α, IL-1β, IL-12, in patients with chronic infections caused by Staphylococcus aureus strains, particularly in the context of the diversification of their Agr system classes. The studies were conducted on adult patients, including 50 patients with chronic suppurative dermatitis, 40 patients with chronic infections of the upper respiratory tract and 25 healthy individuals control group. Blood serum cytokine levels were measured by enzyme-linked immunosorbent assay ELISA. S. aureus was detected in cultures of suppurative dermal exudates or of pharyngeal smears. Classes of Agr systems in the S. aureus strains were identified using polymerase chain reaction PCR. In both groups of patients, on average, levels of IFN-γ were doubled, while levels of IL-17A were increased by 2.5-fold, which, however, was not accompanied by increased levels of TNF-α or IL-12. The data indicate that the development of S. aureus infection among the studied patients was linked to an impoverished cytokine response of monocytes-macrophages, while that induced by the pathogen lymphocytes Th17-Th1 may be responsible for promotion of the chronic inflammatory response. In parallel, no quantitative or qualitative differences were disclosed between cytokine responses manifested by subgroups of patients infected with S. aureus strains belonging to class IV Agr, as compared to patients infected with strains of classes I to III Agr. Nevertheless, in the patients, strains belonging to class IV Agr prevailed, which points to the preferential relationship between the class and the pathogenicity of S. aureus.

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Author: A. Szkaradkiewicz - T. M. Karpiński - A. Zeidler - A. K. Szkaradkiewicz - H. Masiuk - S. Giedrys-Kalemba

Source: https://link.springer.com/







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