Nested pcr reveals elevated over-diagnosis of e. histolytica in barcelona, venezuela. Report as inadecuate




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Beatriz Ahmar ; María E. Rodríguez ; Leonor Mora ; Marcos De Donato ;Investigación Clínica 2012, 53 4

Author: Hectorina Rodulfo

Source: http://www.redalyc.org/articulo.oa?id=372937690006


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Investigación Clínica ISSN: 0535-5133 riclinicas@gmail.com Universidad del Zulia Venezuela Rodulfo, Hectorina; Ahmar, Beatriz; Rodríguez, María E.; Mora, Leonor; De Donato, Marcos Nested PCR reveals elevated over-diagnosis of E.
histolytica in Barcelona, Venezuela. Investigación Clínica, vol.
53, núm.
4, octubre-diciembre, 2012, pp.
365-377 Universidad del Zulia Maracaibo, Venezuela Available in: http:--www.redalyc.org-articulo.oa?id=372937690006 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative Invest Clin 53(4): 365 - 377, 2012 Nested PCR reveals elevated over-diagnosis of E.
histolytica in Barcelona, Venezuela. Hectorina Rodulfo1, Beatriz Ahmar1, María E.
Rodríguez1, Leonor Mora2 y Marcos De Donato1. Laboratorio de Genética Molecular, Instituto de Investigaciones en Biomedicina y Ciencias Aplicadas “Dra.
Susan Tai”, 2Departamento de Bioanálisis. Universidad de Oriente, Núcleo de Sucre.
Cumaná, Venezuela. 1 Keywords: Entamoeba, diagnosis, PCR, E.
histolytica. Abstract.
The aim of this study was to identify the presence of Entamoeba histolytica and E.
dispar by nested PCR in children attending the “Dr.
Luis Razetti” Hospital, Barcelona, Anzoátegui State.
Of the 1,141 fecal samples coproparasitologically evaluated by conventional microscopy, 150 were diagnosed positive for E.
histolytica in 0-10 year-old-children, of both sexes.
The signs, symptoms and a full coproparasitological report were obtained from all of these and nested PCR was performed to identify E. histolytica and E.
dispar.
The conventional microscopy results showed a diagnostic frequency of E.
histolytica in 13.2% of the cases, of which 79.3% were positive only for this pathogen.
However, nested PCR showed that of these, only 28% (42-150) wer...





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