Proteomic analysis reveals the diversity and complexity of membrane proteins in chickpea Cicer arietinum L.Report as inadecuate

Proteomic analysis reveals the diversity and complexity of membrane proteins in chickpea Cicer arietinum L. - Download this document for free, or read online. Document in PDF available to download.

Proteome Science

, 10:59

First Online: 02 October 2012Received: 26 April 2012Accepted: 25 September 2012


BackgroundCompartmentalization is a unique feature of eukaryotes that helps in maintaining cellular homeostasis not only in intra- and inter-organellar context, but also between the cells and the external environment. Plant cells are highly compartmentalized with a complex metabolic network governing various cellular events. The membranes are the most important constituents in such compartmentalization, and membrane-associated proteins play diverse roles in many cellular processes besides being part of integral component of many signaling cascades.

ResultsTo obtain valuable insight into the dynamic repertoire of membrane proteins, we have developed a proteome reference map of a grain legume, chickpea, using two-dimensional gel electrophoresis. MALDI-TOF-TOF and LC-ESI-MS-MS analysis led to the identification of 91 proteins involved in a variety of cellular functions viz., bioenergy, stress-responsive and signal transduction, metabolism, protein synthesis and degradation, among others. Significantly, 70% of the identified proteins are putative integral membrane proteins, possessing transmembrane domains.

ConclusionsThe proteomic analysis revealed many resident integral membrane proteins as well as membrane-associated proteins including those not reported earlier. To our knowledge, this is the first report of membrane proteome from aerial tissues of a crop plant. The findings may provide a better understanding of the biochemical machinery of the plant membranes at the molecular level that might help in functional genomics studies of different developmental pathways and stress-responses.

KeywordsGrain legume Membrane-associated proteins 2-DE Mass spectrometry Transmembrane domain Abbreviations2-DETwo-Dimensional gel Electrophoresis


GRAVYGrand Average of hydropathicity

IDPaseInosine Diphosphatase

IEFIsoElectric Focusing

TMDTransMembrane Domain

SDSSodium Dodecyl Sulphate

PAGEPolyacrylamide Gel Electrophoresis.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-5956-10-59 contains supplementary material, which is available to authorized users.

Doel Ray, Pratigya Subba contributed equally to this work.

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Author: Dinesh Kumar Jaiswal - Doel Ray - Pratigya Subba - Poonam Mishra - Saurabh Gayali - Asis Datta - Subhra Chakraborty - Nira


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