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DARU Journal of Pharmaceutical Sciences

, 20:53

First Online: 15 October 2012Received: 19 July 2012Accepted: 19 July 2012


Background and the purpose of the studyThe cervico-vaginal mucosa which is populated with microflora mostly includes lactobacilli is the portal of entry for sexually transmitted pathogens.

MethodsThe in vitro anti-viral effect of vaginal and non-vaginal lactobacillus was evaluated using single cycle HIV-1 replication and HSV-2 plaque reduction assays. The XTT proliferation assay was used to monitor the cellular toxicity. The in vivo anti-HSV-1 activity was evaluated in BALB-c mouse model by monitoring skin lesion and immune response development.

Results and major conclusionDMEM culture supernatant of L. Gasseri and L. fermentum PH 7.3 did not show toxic effect but inhibited 50% of HIV replication at 12 and 31% concentrations, respectively. Co-culture of L. gasseri 1000 CFU- target cell showed mild cytotoxicity but inhibited 68% of HIV replication. The supernatant of L. crispatus inhibited 50% of HSV replication at 4% and also co-culture of L. gasseri, L. rhamnosus and L. crispatus revokes almost all of the HSV multiplication. Culture supernatants of L. gasseri and L. crispatus had significant virucidal effect against the HIV and HSV and inhibited HSV infection in a stage before viral entry to the target cells. Alive L. gasseri cells showed high potential for inhibiting HSV-1 infection in vivo condition. Current data indicates that lactobacilli supernatant encompasses components with neutralizing activity against HIV and HSV and it would be a determinant factor for viral diseases transmission and promising lead for anti-viral probiotic design.

KeywordsHIV HSV Sexually transmitted disease Lactobacillus Electronic supplementary materialThe online version of this article doi:10.1186-2008-2231-20-53 contains supplementary material, which is available to authorized users.

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Autor: Rezvan Zabihollahi - Elahe Motevaseli - Seyed Mehdi Sadat - Ali Reza Azizi-Saraji - Sogol Asaadi-Dalaie - Mohammad Hossein


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