N-cadherin expression in malignant germ cell tumours of the testisReport as inadecuate

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BMC Clinical Pathology

, 12:19

First Online: 15 October 2012Received: 16 May 2012Accepted: 03 October 2012


BackgroundTesticular germ cell tumours TGCTs are the most common malignancy in young men aged 18–35 years. They are clinically and histologically subdivided into seminomas and non-seminomas. Cadherins are calcium-dependent transmembrane proteins of the group of adhesion proteins. They play a role in the stabilization of cell-cell contacts, the embryonic morphogenesis, in the maintenance of cell polarity and signal transduction. N-cadherin CDH2, the neuronal cadherin, stimulates cell-cell contacts during migration and invasion of cells and is able to suppress tumour cell growth.

MethodsTumour tissues were acquired from 113 male patients and investigated by immunohistochemistry, as were the three TGCT cell lines NCCIT, NTERA-2 and Tcam2. A monoclonal antibody against N-cadherin was used.

ResultsTumour-free testis and intratubular germ cell neoplasias unclassified IGCNU strongly expressed N-cadherin within the cytoplasm. In all seminomas investigated, N-cadherin expression displayed a membrane-bound location. In addition, the teratomas and yolk sac tumours investigated also differentially expressed N-cadherin. In contrast, no N-cadherin could be detected in any of the embryonal carcinomas and chorionic carcinomas examined. This expression pattern was also seen in the investigated mixed tumours consisting of seminomas, teratomas, and embryonal carcinoma.

ConclusionsN-cadherin expression can be used to differentiate embryonal carcinomas and chorionic carcinomas from other histological subtypes of TGCT.

KeywordsN-cadherin Seminoma Embryonal carcinoma Immunohistochemistry TGCT cell lines Electronic supplementary materialThe online version of this article doi:10.1186-1472-6890-12-19 contains supplementary material, which is available to authorized users.

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Author: Felix Bremmer - Bernhard Hemmerlein - Arne Strauss - Peter Burfeind - Paul Thelen - Heinz-Joachim Radzun - Carl Ludwig Beh

Source: https://link.springer.com/

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