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Journal of Ovarian Research

, 5:28

First Online: 15 October 2012Received: 07 September 2012Accepted: 03 October 2012

Abstract

BackgroundTo identifying the effects of DNA methylation and epigenetic factors on the expression of CD133, a cancer stem cell marker, in gynecologic cancer cell lines.

MethodsOvarian cancer cell lines OVCAR-8 and IGROV-1 and an endometrial cancer cell line Ishikawa were treated with 5-aza-2`-deoxycytidine DAC or Trichostatin A TSA. Expression of CD133 was evaluated by quantitative real-time PCR, methylation-specific PCR MSP, reverse transcription- PCR, western blot, and FACS analysis. All results are representative of three independent experiments.

ResultsCD133 mRNA expression varied among the different cell lines; the weakest expression was observed in OVCAR-8 cells, while it was strongly expressed in Ishikawa cells. The degree of methylation of the CD133 P2 promoter was 61% in OVCAR-8 cells, 53% in IGROV-1 cells, and 43% in Ishikawa cells. CD133 expression was increased at both the mRNA and protein level after DAC treatment. On the contrary, CD133 mRNA expression decreased after TSA treatment decreased in all cell lines except OVCAR-8. In addition, MSP of the CD133 P2 promoter revealed that methylation was reduced after treatment with either DAC or TSA.

ConclusionsThe expression of the CD133 antigen in primary ovarian and endometrial cancer cell lines is regulated by epigenetics, as indicated by its increased expression following DAC treatment and irregular expression pattern followed by TSA treatment. In addition, the expression of CD133 was negatively correlated with the degree of methylation of the CD133 P2 promoter.

KeywordsCancer stem cell CD133 Epigenetics 5`-aza-2`-deoxycytidine Trichostatin A Electronic supplementary materialThe online version of this article doi:10.1186-1757-2215-5-28 contains supplementary material, which is available to authorized users.

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Autor: Kyung-Jin Min - Kyeong A So - Yung-Taek Ouh - Jin-Hwa Hong - Jae-Kwan Lee

Fuente: https://link.springer.com/



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