Lactoferrin restrains allergen-induced pleurisy in miceReportar como inadecuado

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Inflammation Research

, Volume 61, Issue 11, pp 1247–1255

First Online: 19 July 2012Received: 26 December 2011Revised: 06 June 2012Accepted: 27 June 2012


ObjectivesThe aim of this study was to assess the utility of lactoferrin LF, a natural immunomodulator, to restrain allergen-induced pleurisy in mice.

Material and subjectsBALB-c female mice, 8- to 10-week old, weighing 24 g on average, were used.

TreatmentMice were immunized intraperitoneally with 50 μg of ovalbumin OVA and the pleurisy was elicited 14 days later by intrapleural injection of 12.5 μg of OVA. LF was given 24 and 3 h before elicitation of the allergic reaction.

MethodsThe cytokine levels in the pleural exudates were measured by immunoassays. The blood and pleural exudates smears were stained with Giemsa and May-Grünwald reagents and reviewed histologically. Lung sections were stained with eosin and hematoxylin for histological evaluation.

ResultsLactoferrin significantly decreased manifestation of pleurisy induced by OVA in a sensitized mouse model. In particular, the percentages of eosinophils in blood and pleural exudates were strongly diminished. The histological analysis of lungs revealed that LF diminished the development of pathological lesions, such as pulmonary edema, diffuse alveolar hemorrhage and hemosiderosis, which were found in the lungs after injection of the eliciting dose of OVA. LF also decreased the level of IL-5 secreted into the pleural fluid.

ConclusionsThis is a first demonstration that LF significantly decreases antigen-specific pleurisy in a sensitized mouse model.

KeywordsAllergy Buccal Lactoferrin Mice Pleurisy Responsible Editor: Ian Ahnfelt-Rønne.

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Autor: Michał Zimecki - Jolanta Artym - Maja Kocięba - Katarzyna Kaleta-Kuratewicz - Marian L. Kruzel


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