Intact skin and not stripped skin is crucial for the safety and efficacy of peanut epicutaneous immunotherapy EPIT in miceReportar como inadecuado




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Clinical and Translational Allergy

, 2:22

Advances in food allergy

Abstract

BackgroundEpicutaneous immunotherapy EPIT on intact skin with an epicutaneous delivery system has already been used in preclinical and clinical studies. In epicutaneous vaccination and immunotherapy, the stripping of skin before application of the allergen is suggested to facilitate the passage of allergen through immune cells.

ObjectivesThe aim of this study was to compare the immunological response induced by EPIT performed on intact and stripped skin in a mouse model of peanut allergy.

MethodsAfter oral sensitization with peanut and cholera toxin, BALB-c mice were epicutaneously treated using an epicutaneous delivery system Viaskin® DBV Technologies, Paris applied either on intact skin or on stripped skin. Following EPIT, mice received an exclusive oral peanut regimen, aimed at triggering esophageal and jejunal lesions. We assessed eosinophil infiltration by histology, mRNA expression in the esophagus, antibody levels and peripheral T-cell response.

ResultsEPIT on intact skin significantly reduced Th2 immunological response IgE response and splenocyte secretion of Th2 cytokines as well as esophageal eosinophilia 2.7 ± 0.9, compared to Sham 19.9 ± 1.5, p < 0.01, mRNA expression of Th2 cytokines in tissue and intestinal villus sub-atrophia 2.9 ± 0.2 vs Sham, 2.1 ± 0.2, p < 0.05. By contrast, EPIT on stripped skin reinforced Th2 systemic immunological response as well as eosinophil infiltration 26.8 ± 15.1, mRNA expression of Th2 cytokines and duodenal villus-crypt-ratio 2.4 ± 0.3.

ConclusionsEpicutaneous allergen-specific immunotherapy needs the integrity of superficial layers of the stratum corneum to warranty safety of treatment and to induce a tolerogenic profile of the immune response.

KeywordsFood allergy Immunotherapy Epicutaneous Peanut AbbreviationsDCsDendritic Cells

EPITEpicutaneous Immunotherapy

Ig EG1, G2a, Immunoglobulin type E, G1, G2a

RT-qPCRReverse Transcriptase-quantitative-Polymerase Chain Reaction

Th2 cellsT helper type 2 cells.

Electronic supplementary materialThe online version of this article doi:10.1186-2045-7022-2-22 contains supplementary material, which is available to authorized users.

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Autor: Lucie Mondoulet - Vincent Dioszeghy - Emilie Puteaux - Mélanie Ligouis - Véronique Dhelft - Franck Letourneur - Christophe

Fuente: https://link.springer.com/







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