Mitochondrial peroxidase TPx-2 is not essential in the blood and insect stages of Plasmodium bergheiReportar como inadecuado




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Parasites and Vectors

, 5:252

First Online: 12 November 2012Received: 02 July 2012Accepted: 08 November 2012

Abstract

BackgroundMalaria parasites actively proliferate in the body of their vertebrate and insect hosts, and are subjected to the toxic effects of reactive oxygen species. The antioxidant defenses of malaria parasites are considered to play essential roles in their survival and are thus considered promising targets for intervention. We sought to identify the cellular function of thioredoxin peroxidase-2 TPx-2, which is expressed in the mitochondria, by disrupting the TPx-2 gene pbtpx-2 of the rodent malaria parasite Plasmodium berghei.

FindingsIn three independent experiments, two disruptant populations TPx-2 KO and three wild-type parasite populations with pyrimethamine resistance dhfr-ts-mt at the DHFR-TS locus and intact pbtpx-2 TPx-2 WT were obtained and cloned. Null expression of TPx-2 in the KO population was confirmed by RT-PCR and Western blot analyses. The TPx-2 KO parasite developed normally in mouse erythrocytes and multiplied at a rate similar to that of the TPx-2 WT parasite during the experimental period. The peak period of gametocytemia was delayed by 1 day in the TPx-2 KO compared with that of the TPx-2 WT and the parent parasite, however, the highest gametocyte number was comparable. The number of midgut oocysts in the TPx-2 KO at 14 days post feeding was comparable to that of the TPx-2 WT.

ConclusionsThe present finding suggests that mitochondrial Prx TPx-2 is not essential for asexual and the insect stage development of the malaria parasite.

KeywordsPeroxiredoxin Plasmodium berghei Thioredoxin peroxidase AbbreviationsGSHGlutathione

PrxPeroxiredoxin

TPxThioredoxin peroxidase

ROSReactive oxygen species.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-3305-5-252 contains supplementary material, which is available to authorized users.

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Autor: Hirono Masuda-Suganuma - Miho Usui - Shinya Fukumoto - Noboru Inoue - Shin-ichiro Kawazu

Fuente: https://link.springer.com/







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