MicroRNA expression profile associated with response to neoadjuvant chemoradiotherapy in locally advanced rectal cancer patientsReportar como inadecuado




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Radiation Oncology

, 7:195

First Online: 20 November 2012Received: 08 August 2012Accepted: 11 November 2012

Abstract

BackgroundRectal cancer accounts for approximately one third of all colorectal cancers CRC, which belong among leading causes of cancer deaths worldwide. Standard treatment for locally advanced rectal cancer cT3-4 and-or cN+ includes neoadjuvant chemoradiotherapy with fluoropyrimidines capecitabine or 5-fluorouracil followed by radical surgical resection. Unfortunately, a significant proportion of tumors do not respond enough to the neoadjuvant treatment and these patients are at risk of relapse. MicroRNAs miRNAs are small non-coding RNAs playing significant roles in the pathogenesis of many cancers including rectal cancer. MiRNAs could present the new predictive biomarkers for rectal cancer patients.

MethodsWe selected 20 patients who underwent neoadjuvant chemoradiotherapy for advanced rectal cancer and whose tumors were classified as most sensitive or resistant to the treatment. These two groups were compared using large-scale miRNA expression profiling.

ResultsExpression levels of 8 miRNAs significantly differed between two groups. MiR-215, miR-190b and miR-29b-2* have been overexpressed in non-responders, and let-7e, miR-196b, miR-450a, miR-450b-5p and miR-99a* have shown higher expression levels in responders. Using these miRNAs 9 of 10 responders and 9 of 10 non-responders p < 0.05 have been correctly classified.

ConclusionsOur pilot study suggests that miRNAs are part of the mechanisms that are involved in response of rectal cancer to the chemoradiotherapy and that miRNAs may be promising predictive biomarkers for such patients. In most miRNAs we identified miR-215, miR-99a*, miR-196b, miR-450b-5p and let-7e, the connection between their expression and radioresistance or chemoresistance to inhibitors of thymidylate synthetase was already established.

KeywordsRectal cancer microRNA Prediction Neoadjuvant chemoradiotherapy Radiation Capecitabine 5-fluorouracil Thymidylate synthetase Electronic supplementary materialThe online version of this article doi:10.1186-1748-717X-7-195 contains supplementary material, which is available to authorized users.

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Autor: Marek Svoboda - Jiri Sana - Pavel Fabian - Ilona Kocakova - Jana Gombosova - Jana Nekvindova - Lenka Radova - Rostislav Vy

Fuente: https://link.springer.com/







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