Genetic characterization of a reptilian calicivirus Cro1Report as inadecuate

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Virology Journal

, 9:297

First Online: 29 November 2012Received: 15 August 2012Accepted: 22 November 2012


BackgroundVesiviruses in the family Caliciviridae infect a broad range of animal hosts including mammals, birds, fish, amphibians and reptiles. The vesivirus Cro1 strains were isolated from diseased snakes in the San Diego zoo in 1978 and reported as the first caliciviruses found in reptiles. The goal of this study was to characterize the Cro1 strain 780032I that was isolated in cell culture from a rock rattlesnake Crotalus lepidus in the original outbreak.

ResultsWe re-amplified the original virus stock in Vero cells, and determined its full-length genome sequence. The Cro1 genome is 8296 nucleotides nt in length and has a typical vesivirus organization, with three open reading frames ORF, ORF1 5643 nt, ORF2 2121 nt, and ORF3 348 nt encoding a nonstructural polyprotein, the major capsid protein precursor, and a minor structural protein, respectively. Phylogenetic analysis of the full-length genome sequence revealed that the Cro1 virus clustered most closely with the VESV species of the genus Vesivirus, but was genetically distinct 82-83% identities with closest strains.

ConclusionsThis is the first description of a full-length genome sequence from a reptile calicivirus Cro1. The availability of the Cro1 genome sequence should facilitate investigation of the molecular mechanisms involved in Cro1 virus evolution and host range.

KeywordsReptile calicivirus Cro1 Complete genome Vesivirus phylogeny Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-9-297 contains supplementary material, which is available to authorized users.

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Author: Carlos Sandoval-Jaime - Gabriel I Parra - Alvin W Smith - Kim Y Green - Stanislav V Sosnovtsev


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