Production of the Bacillus licheniformis SubC protease using Lactococcus lactis NICE expression systemReportar como inadecuado

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Biomedical and Life Sciences


In this work the subC gene from Bacillus licheniformis encoding subtilisin was cloned into the nisin-controlled expression NICE vectors pNZ8048 and pNZ8148 with or without the signal peptide SP Usp45 directing extracellular secretion via Sec machinery. Extracellular protease production and activity was tested using Lactococcus lactis NZ9000 as host, which could be used for rennet production. The efficiency of protein production was tested using purified nisin and the supernatant of L. lactis NZ970 nisin producer. Similar results were obtained for 1 ng-ml nisin and 10 000 diluted supernatant. SP Usp45 signal peptide effectively directed extracellular localization of active and stable protease. SubC signal for extracellular localization in B. licheniformis, was also recognized by L. lactis Sec pathway, although with lower efficiency, as shown by a 3-fold lower protease activity in the medium. Protease production and activity was optimized using parameters such as induction time, nutrients glucose, casitone supplementation during growth or protease stabilization by calcium ions. The results were also verified in fed-batch bioreactor for further scale-up of the expression system.

KeywordsLactic acid bacteria Lactococcus lactis Nisin-controlled expression system NICE Bacillus licheniformis SubC protease Electronic supplementary materialThe online version of this article doi:10.1186-2193-1801-1-54 contains supplementary material, which is available to authorized users.

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Autor: Aleksandra M Mirończuk - Anna Krasowska - Anna Murzyn - Małgorzata Płachetka - Marcin Łukaszewicz


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